Jing Shao1, Youming Peng2, Liyu He1, Hong Liu1, Xian Chen1, Xiaofei Peng1. 1. Nephrology Department, 2nd Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification, People's Republic of China. 2. Nephrology Department, 2nd Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification, People's Republic of China. Electronic address: ympeng2011@aliyun.com.
Abstract
BACKGROUND: IgA nephropathy (IgAN) is the most common type of primary glomerulonephritis in the world. Hot pepper is the most favorite vegetable for Chinese in Hunan and Sichuan provinces. It can be assumed that capsaicin, the active ingredient of hot pepper, is a possible risk factor in diet in the pathogenesis of IgAN. METHODS: 22 subjects, including 11 IgAN patients and 11 non-IgAN patients were enrolled in this study. Tonsillar mononuclear cells were isolated and cultured for 3days with or without capsaicin. RESULTS: In the absence and presence of capsaicin, the BAFF expression and IgA1 secretion were higher in IgAN patients than that in non-IgAN patients, meanwhile, the gene expression of C1GALT1 and Cosmc and IgA1 O-glycosylation level were significantly lower. In IgAN group, coincubated with capsaicin, IgA1 and BAFF secretion and BAFF expression by TMCs were significantly higher than that without capsaicin, furthermore, the level of mRNA encoding C1GALT1 and Cosmc and the level of IgA1 O-glycosylation were evidently lower. CONCLUSION: Capsaicin may induce IgA1 secretion by activating BAFF expression, and bring to aberrantly IgA1 O-glycosylation by suppressing C1GALT1 and Cosmc expression. Therefore, to limit the consumption of hot pepper would be beneficial to patients with IgA nephropathy.
BACKGROUND:IgA nephropathy (IgAN) is the most common type of primary glomerulonephritis in the world. Hot pepper is the most favorite vegetable for Chinese in Hunan and Sichuan provinces. It can be assumed that capsaicin, the active ingredient of hot pepper, is a possible risk factor in diet in the pathogenesis of IgAN. METHODS: 22 subjects, including 11 IgANpatients and 11 non-IgANpatients were enrolled in this study. Tonsillar mononuclear cells were isolated and cultured for 3days with or without capsaicin. RESULTS: In the absence and presence of capsaicin, the BAFF expression and IgA1 secretion were higher in IgANpatients than that in non-IgANpatients, meanwhile, the gene expression of C1GALT1 and Cosmc and IgA1 O-glycosylation level were significantly lower. In IgAN group, coincubated with capsaicin, IgA1 and BAFF secretion and BAFF expression by TMCs were significantly higher than that without capsaicin, furthermore, the level of mRNA encoding C1GALT1 and Cosmc and the level of IgA1 O-glycosylation were evidently lower. CONCLUSION:Capsaicin may induce IgA1 secretion by activating BAFF expression, and bring to aberrantly IgA1 O-glycosylation by suppressing C1GALT1 and Cosmc expression. Therefore, to limit the consumption of hot pepper would be beneficial to patients with IgA nephropathy.
Authors: Philip Chiu-Tsun Tang; Alex Siu-Wing Chan; Cai-Bin Zhang; Cristina Alexandra García Córdoba; Ying-Ying Zhang; Ka-Fai To; Kam-Tong Leung; Hui-Yao Lan; Patrick Ming-Kuen Tang Journal: Front Med (Lausanne) Date: 2021-02-25