Marian Kacerovsky1,2, Lubomira Tothova3,4, Ramkumar Menon5, Barbora Vlkova3,4, Ivana Musilova2, Helena Hornychova6, Martin Prochazka7, Peter Celec3,4. 1. a Biomedical Research Center, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic. 2. b Department of Obstetrics and Gynecology, Faculty of Medicine Hradec Kralove , Charles University in Prague , Prague , Czech Republic. 3. c Institute of Molecular Biomedicine, Comenius University in Bratislava , Bratislava , Slovak Republic. 4. d Center for Molecular Medicine, Slovak Academy of Sciences , Bratislava , Slovakia. 5. e Department of Obstetrics and Gynecology , The University of Texas Medical Branch at Galveston , Galveston , USA. 6. f Fingerland's Department of Pathology, Faculty of Medicine Hradec Kralove , Charles University in Prague, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic , and. 7. g Department of Obstetrics and Gynecology , Palacky University Olomouc, University Hospital Olomouc , Olomouc , Czech Republic.
Abstract
OBJECTIVE: To determine amniotic fluid total antioxidant capacity (TAC), ferric-reducing antioxidant power (FRAP) and thiobarbituric acid-reacting substances (TBARS), markers of oxidative stress, in pregnancies complicated by preterm prelabor rupture of membranes (pPROM) and their correlation to microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA). METHODS: One-hundred thirty-eight women with singleton pregnancies complicated by pPROM were included in this study. Amniotic fluid was collected by transabdominal amniocentesis at the time of admission and amniotic fluid concentrations of TAC, FRAP and TBARS were measured. RESULT: The presence of MIAC and/or HCA did not show any significant differences in the amniotic fluid TAC, FRAP and TBARS concentrations. Positive correlations between gestational age at sampling and amniotic fluid TAC and FRAP concentrations were found (TAC: rho = 0.32; p = 0.0002; FRAP: rho = 0.36; p < 0.0001). A negative correlation between gestation age at sampling and amniotic fluid TBARS concentrations was identified (rho = -0.25; p = 0.004). CONCLUSIONS: Oxidative stress is associated with pPROM as indicated by the presence of markers tested in the amniotic fluid; however, oxidative stress markers tested are not influenced by the presence of MIAC or HCA.
OBJECTIVE: To determine amniotic fluid total antioxidant capacity (TAC), ferric-reducing antioxidant power (FRAP) and thiobarbituric acid-reacting substances (TBARS), markers of oxidative stress, in pregnancies complicated by preterm prelabor rupture of membranes (pPROM) and their correlation to microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA). METHODS: One-hundred thirty-eight women with singleton pregnancies complicated by pPROM were included in this study. Amniotic fluid was collected by transabdominal amniocentesis at the time of admission and amniotic fluid concentrations of TAC, FRAP and TBARS were measured. RESULT: The presence of MIAC and/or HCA did not show any significant differences in the amniotic fluid TAC, FRAP and TBARS concentrations. Positive correlations between gestational age at sampling and amniotic fluid TAC and FRAP concentrations were found (TAC: rho = 0.32; p = 0.0002; FRAP: rho = 0.36; p < 0.0001). A negative correlation between gestation age at sampling and amniotic fluid TBARS concentrations was identified (rho = -0.25; p = 0.004). CONCLUSIONS: Oxidative stress is associated with pPROM as indicated by the presence of markers tested in the amniotic fluid; however, oxidative stress markers tested are not influenced by the presence of MIAC or HCA.
Authors: Ramkumar Menon; Elizabeth A Bonney; Jennifer Condon; Sam Mesiano; Robert N Taylor Journal: Hum Reprod Update Date: 2016-06-30 Impact factor: 15.610
Authors: Ivana Musilova; Lenka Pliskova; Romana Gerychova; Petr Janku; Ondrej Simetka; Petr Matlak; Bo Jacobsson; Marian Kacerovsky Journal: PLoS One Date: 2017-12-12 Impact factor: 3.240