Fátima Pérez de Heredia1, Marta Garaulet2, Sonia Gómez-Martínez3, Ligia E Díaz3, Julia Wärnberg4, Odysseas Androutsos5, Nathalie Michels6, Christina Breidenassel7, Magdalena Cuenca-García8, Inge Huybrechts9, Frédéric Gottrand10, Marika Ferrari11, Alba M Santaliestra-Pasías12, Anthony Kafatos13, Denes Molnár14, Michael Sjöstrom4, Kurt Widhalm15, Luis A Moreno16, Ascensión Marcos3. 1. Immunonutrition Research Group, Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition (ICTAN), Spanish National Research Council (CSIC), Madrid, Spain; School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool, United Kingdom. Electronic address: f.perezdeherediabenedicte@ljmu.ac.uk. 2. Department of Physiology, Faculty of Biology, University of Murcia, Murcia, Spain. 3. Immunonutrition Research Group, Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition (ICTAN), Spanish National Research Council (CSIC), Madrid, Spain. 4. Unit for Preventive Nutrition, Department of Biosciences and Nutrition at Novum, Karolinska Institutet, Stockholm, Sweden. 5. Department of Nutrition and Dietetics, University of Harokopio, Athens, Greece. 6. Department of Public Health, Faculty of Medicine and Health Sciences, University of Ghent, UZ-Ghent, Gent, Belgium. 7. Department of Nutrition and Food Science, University of Bonn, Bonn, Germany; ImFINE Research Group, Department of Health and Human Performance, Faculty of Physical Activity and Sports Sciences-INEF, Technical University of Madrid, Madrid, Spain. 8. Department of Medical Physiology, School of Medicine, University of Granada, Granada, Spain. 9. Dietary Exposure Assessment Group, International Agency for Research on Cancer, Lyon, France. 10. Hôpital J de Flandre CHRU de Lille, Inserm U995, Faculté de Médecine, Université de Lille 2, Lille, France. 11. National Research Institute on Food and Nutrition, Rome, Italy. 12. GENUD (Growth, Exercise, Nutrition and Development) Research Group, University of Zaragoza, Zaragoza, Spain. 13. Preventive Medicine and Nutrition Unit, School of Medicine, University of Crete, Heraklion, Greece. 14. Department of Pediatrics, University of Pécs, Pécs, Hungary. 15. Department of Pediatrics, Division of Clinical Nutrition and Prevention, Medical University of Vienna, Vienna, Austria. 16. GENUD (Growth, Exercise, Nutrition and Development) Research Group, University of Zaragoza, Zaragoza, Spain; Department of Preventive Medicine, University of São Paulo, São Paulo, Brazil.
Abstract
BACKGROUND: Sleep patterns face important changes during adolescence. This can have implications for the immune system, which is regulated by the sleep-wake cycle; however, most studies relating sleep and immune system have been conducted on adults. OBJECTIVE: To study the relationships between sleep duration, immune cell counts, and cytokines in European adolescents participating in the HELENA Cross-Sectional Study. METHODS: Adolescents (12.5-17.5 years; n = 933; 53.9% girls) were grouped according to self-reported sleep duration into <8, 8-8.9 and ≥9 h/night. Blood samples were collected in the morning after an overnight fast to analyze counts of white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, basophils, the lymphocyte subsets CD3(+), CD4(+), CD8(+), CD45RA(+), CD45RO(+), CD3(-)CD16(+)56(+) and CD19(+), and concentrations of cortisol, CRP, IL-1, IL-2, IL-4, IL-5, IL-6, IL-10, TNF-α and IFN-γ. Pro-/anti-inflammatory and Th1/Th2 cytokine ratios were calculated. Immune parameters were correlated to sleep duration and compared between the three groups. RESULTS: Sleep duration was negatively associated with cortisol levels and WBC, neutrophil, monocyte, CD4(+) and CD4(+)CD45RO(+) counts; in girls it is also negatively associated with IL-5 and IL-6 levels. The 8-8.9 h/night group presented the highest IL-4 values and the lowest pro-/anti-inflammatory and Th1/Th2 cytokine ratios. CONCLUSION: A sleep duration of 8-8.9 h/night was associated with a healthier immune profile in our adolescents.
BACKGROUND: Sleep patterns face important changes during adolescence. This can have implications for the immune system, which is regulated by the sleep-wake cycle; however, most studies relating sleep and immune system have been conducted on adults. OBJECTIVE: To study the relationships between sleep duration, immune cell counts, and cytokines in European adolescents participating in the HELENA Cross-Sectional Study. METHODS: Adolescents (12.5-17.5 years; n = 933; 53.9% girls) were grouped according to self-reported sleep duration into <8, 8-8.9 and ≥9 h/night. Blood samples were collected in the morning after an overnight fast to analyze counts of white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, basophils, the lymphocyte subsets CD3(+), CD4(+), CD8(+), CD45RA(+), CD45RO(+), CD3(-)CD16(+)56(+) and CD19(+), and concentrations of cortisol, CRP, IL-1, IL-2, IL-4, IL-5, IL-6, IL-10, TNF-α and IFN-γ. Pro-/anti-inflammatory and Th1/Th2 cytokine ratios were calculated. Immune parameters were correlated to sleep duration and compared between the three groups. RESULTS: Sleep duration was negatively associated with cortisol levels and WBC, neutrophil, monocyte, CD4(+) and CD4(+)CD45RO(+) counts; in girls it is also negatively associated with IL-5 and IL-6 levels. The 8-8.9 h/night group presented the highest IL-4 values and the lowest pro-/anti-inflammatory and Th1/Th2 cytokine ratios. CONCLUSION: A sleep duration of 8-8.9 h/night was associated with a healthier immune profile in our adolescents.
Authors: Shalini Paruthi; Lee J Brooks; Carolyn D'Ambrosio; Wendy A Hall; Suresh Kotagal; Robin M Lloyd; Beth A Malow; Kiran Maski; Cynthia Nichols; Stuart F Quan; Carol L Rosen; Matthew M Troester; Merrill S Wise Journal: J Clin Sleep Med Date: 2016-11-15 Impact factor: 4.062
Authors: Md Dilshad Manzar; Mohammad Muntafa Rajput; Wassilatul Zannat; Unaise Abdul Hameed; Muhammed Deeb Al-Jarrah; David Warren Spence; Seithikurippu R Pandi-Perumal; Ahmed S BaHammam; M Ejaz Hussain Journal: Sleep Breath Date: 2015-09-09 Impact factor: 2.816