Dong Wan Sohn1, Woong Jin Bae2, Hyo Sin Kim1, Sun Wook Kim1, Sae Woong Kim3. 1. Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea. 2. Catholic Integrative Medicine Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea. 3. Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea. Electronic address: ksw1227@catholic.ac.kr.
Abstract
OBJECTIVE: To evaluate the potential of anthocyanin as a supplement for the treatment of Peyronie disease (PD) by examining the anti-inflammatory and antifibrosis activities of anthocyanin in a PD animal model. MATERIALS AND METHODS: We performed the preliminary experiment to confirm the plaque formation of tunica albuginea (TA). Six animals received fibrin (TISSEEL VH Sealer; Baxter, Glendale, CA; 30 μL each of human fibrin and thrombin solutions) injections into the TA. After 2 weeks, PD-like plaque formation was confirmed by Masson trichrome staining in preliminary experimental animal group. A PD rat model was made by intratunical injection of fibrin. Experimental animals were divided into 3 groups as follows: control group (n = 8), PD group (n = 8), and anthocyanin-treated group (n = 8) that received anthocyanin at 50 mg/kg twice a day for 4 weeks using orogastric tubes. After 4 weeks, penile tissue was collected to perform Masson trichrome and transforming growth factor (TGF)-β1 staining. RESULTS: We confirmed PD-like plaque formation by Masson trichrome stain 2 weeks after fibrin injection. The ratio of smooth muscle cells in the corpus cavernosum in the PD group was significantly lower than that of the control group (P <.05). The PD group showed strong TGF-β1 immunoreactivity with increased expression in the collagenous connective tissues and fibroblasts around the TA. CONCLUSION: We confirmed PD-like plaque formation after 2 weeks of fibrin injections into the TA. This is the first study to suggest that anthocyanin extracted from black soybean may have anti-inflammatory and antifibrotic effects for penile plaque formation in rat PD models.
OBJECTIVE: To evaluate the potential of anthocyanin as a supplement for the treatment of Peyronie disease (PD) by examining the anti-inflammatory and antifibrosis activities of anthocyanin in a PD animal model. MATERIALS AND METHODS: We performed the preliminary experiment to confirm the plaque formation of tunica albuginea (TA). Six animals received fibrin (TISSEEL VH Sealer; Baxter, Glendale, CA; 30 μL each of human fibrin and thrombin solutions) injections into the TA. After 2 weeks, PD-like plaque formation was confirmed by Masson trichrome staining in preliminary experimental animal group. A PDrat model was made by intratunical injection of fibrin. Experimental animals were divided into 3 groups as follows: control group (n = 8), PD group (n = 8), and anthocyanin-treated group (n = 8) that received anthocyanin at 50 mg/kg twice a day for 4 weeks using orogastric tubes. After 4 weeks, penile tissue was collected to perform Masson trichrome and transforming growth factor (TGF)-β1 staining. RESULTS: We confirmed PD-like plaque formation by Masson trichrome stain 2 weeks after fibrin injection. The ratio of smooth muscle cells in the corpus cavernosum in the PD group was significantly lower than that of the control group (P <.05). The PD group showed strong TGF-β1 immunoreactivity with increased expression in the collagenous connective tissues and fibroblasts around the TA. CONCLUSION: We confirmed PD-like plaque formation after 2 weeks of fibrin injections into the TA. This is the first study to suggest that anthocyanin extracted from black soybean may have anti-inflammatory and antifibrotic effects for penile plaque formation in ratPD models.