| Literature DB >> 25155958 |
J Michael Gee1, Nathan A Smith2, Fernando R Fernandez3, Michael N Economo4, Daniela Brunert4, Markus Rothermel4, S Craig Morris5, Amy Talbot6, Sierra Palumbos6, Jennifer M Ichida7, Jason D Shepherd7, Peter J West8, Matt Wachowiak4, Mario R Capecchi9, Karen S Wilcox8, John A White10, Petr Tvrdik11.
Abstract
New strategies for introducing genetically encoded activity indicators into animal models facilitate the investigation of nervous system function. We have developed the PC::G5-tdT mouse line that expresses the GCaMP5G calcium indicator in a Cre-dependent fashion. Instead of targeting the ROSA26 locus, we inserted the reporter cassette nearby the ubiquitously expressed Polr2a gene without disrupting locus integrity. The indicator was tagged with IRES-tdTomato to aid detection of positive cells. This reporter system is effective in a wide range of developmental and cellular contexts. We recorded spontaneous cortical calcium waves in intact awake newborns and evaluated concentration-dependent responses to odorants in the adult olfactory bulb. Moreover, PC::G5-tdT effectively reports intracellular calcium dynamics in somas and fine processes of astrocytes and microglial cells. Through electrophysiological and behavioral analyses, we determined that GCaMP5G expression had no major impact on nervous system performance. PC::G5-tdT will be instrumental for a variety of brain mapping experiments.Entities:
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Year: 2014 PMID： 25155958 PMCID： PMC4156920 DOI： 10.1016/j.neuron.2014.07.024
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173