Literature DB >> 25154385

Optimization of ELFSE DNA sequencing with EOF counterflow and microfluidics.

Max A Fahrenkopf1, Tamal Mukherjee, B Erik Ydstie, James W Schneider.   

Abstract

We present a nonlinear optimization study of different implementations of the DNA electrophoretic method "End-labeled Free-solution Electrophoresis" in commercial capillary electrophoresis systems and microfluidics to improve the time required for readout. Here, the effect of electro-osmotic counterflows and snap-shot detection are considered to allow for detection of peaks soon after they are electorphoretically resolved. Using drag tags available in micelle form, we identify a design capable of sequencing 600 bases in 2.8 min.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  DNA electrophoresis; DNA sequencing; ELFSE; Gel-free; Rapid

Mesh：

Substances：

Year:  2014        PMID： 25154385      PMCID： PMC4504435          DOI： 10.1002/elps.201400266

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  10 in total

1.  Separating DNA sequencing fragments without a sieving matrix.

Authors:  H Ren; A E Karger; F Oaks; S Menchen; G W Slater; G Drouin
Journal:  Electrophoresis       Date:  1999-09       Impact factor: 3.535

2.  Band spreading in two-dimensional microchannel turns for electrokinetic species transport

Authors: 
Journal:  Anal Chem       Date:  2000-11-01       Impact factor: 6.986

3.  System-oriented dispersion models of general-shaped electrophoresis microchannels.

Authors:  Yi Wang; Qiao Lin; Tamal Mukherjee
Journal:  Lab Chip       Date:  2004-07-30       Impact factor: 6.799

4.  A 265-base DNA sequencing read by capillary electrophoresis with no separation matrix.

Authors:  Jennifer Coyne Albrecht; Jennifer S Lin; Annelise E Barron
Journal:  Anal Chem       Date:  2010-12-23       Impact factor: 6.986

5.  A theoretical study of the possible use of electroosmotic flow to extend the read length of DNA sequencing by end-labeled free solution electrophoresis.

Authors:  Laurette C McCormick; Gary W Slater
Journal:  Electrophoresis       Date:  2006-05       Impact factor: 3.535

6.  Identification of PCR products using PNA amphiphiles in micellar electrokinetic chromatography.

Authors:  Shane T Grosser; Jeffrey M Savard; James W Schneider
Journal:  Anal Chem       Date:  2007-11-20       Impact factor: 6.986

7.  Sequencing of DNA by free-solution capillary electrophoresis using a genetically engineered protein polymer drag-tag.

Authors:  Robert J Meagher; Jong-In Won; Jennifer A Coyne; Jennifer Lin; Annelise E Barron
Journal:  Anal Chem       Date:  2008-03-05       Impact factor: 6.986

8.  Length-dependent DNA separations using multiple end-attached peptide nucleic acid amphiphiles in micellar electrokinetic chromatography.

Authors:  Jeffrey M Savard; Shane T Grosser; James W Schneider
Journal:  Electrophoresis       Date:  2008-07       Impact factor: 3.535

9.  Modeling and Global Optimization of DNA separation.

Authors:  Max A Fahrenkopf; B Erik Ydstie; Tamal Mukherjee; James W Schneider
Journal:  Comput Chem Eng       Date:  2014-05-07       Impact factor: 3.845

10.  Microchip devices for high-efficiency separations.

Authors:  C T Culbertson; S C Jacobson; J M Ramsey
Journal:  Anal Chem       Date:  2000-12-01       Impact factor: 6.986
  10 in total
  2 in total

Review 1.  Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009-2014).

Authors:  Brandon C Durney; Cassandra L Crihfield; Lisa A Holland
Journal:  Anal Bioanal Chem       Date:  2015-05-03       Impact factor: 4.142

Review 2.  A Review of Optical Imaging Technologies for Microfluidics.

Authors:  Pan Zhou; Haipeng He; Hanbin Ma; Shurong Wang; Siyi Hu
Journal:  Micromachines (Basel)       Date:  2022-02-08       Impact factor: 2.891
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.