Shenxu Wang1,2, Sai Ma3, Xiujuan Li3, Zengfu Xue4, Xiaotian Zhang3, Weiwei Fan3, Yongzhan Nie4, Kaichun Wu4, Xiaoyuan Chen5, Feng Cao1,3. 1. Cardiology Department, Chinese PLA General Hospital, Beijing, China. 2. Cardiology Department, No. 150 Hospital of PLA, LuoYang, Henan, China. 3. Cardiology Department, Xijing Hospital, Xi'an, Shaanxi, China. 4. Digestive Disease Department, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China. 5. Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, China.
Abstract
PURPOSE: To investigate the effect of cisplatin on the growth and metastasis abilities of lung cancer stem cells (CSCs) via molecular imaging. MATERIALS AND METHODS: The expression changes of lung CSCs cell marker in A549-Luc-C8 human non-small-cell lung cancer (NSCLC) cell line with or without cisplatin treatment were detected by flow cytometry. The tumorigenesis and metastasis abilities of A549-Luc-C8 cells were monitored both in vitro and in vivo, and the mechanism was assessed by gene sequencing. RESULTS: About 1%-2% of CSCs were detected in A549-Luc-C8 cells and decreased CSCs percentage was observed after cisplatin treatment. Attenuated tumorigenesis and metastasis abilities of A549-Luc-C8 cells were found in cisplatin treated group. CONCLUSIONS: Decreased percentage of CSCs in A549-Luc-C8 cells can be induced by cisplatin treatment, which may partly be attributed to the attenuated expression of growth factors.
PURPOSE: To investigate the effect of cisplatin on the growth and metastasis abilities of lung cancer stem cells (CSCs) via molecular imaging. MATERIALS AND METHODS: The expression changes of lung CSCs cell marker in A549-Luc-C8 human non-small-cell lung cancer (NSCLC) cell line with or without cisplatin treatment were detected by flow cytometry. The tumorigenesis and metastasis abilities of A549-Luc-C8 cells were monitored both in vitro and in vivo, and the mechanism was assessed by gene sequencing. RESULTS: About 1%-2% of CSCs were detected in A549-Luc-C8 cells and decreased CSCs percentage was observed after cisplatin treatment. Attenuated tumorigenesis and metastasis abilities of A549-Luc-C8 cells were found in cisplatin treated group. CONCLUSIONS: Decreased percentage of CSCs in A549-Luc-C8 cells can be induced by cisplatin treatment, which may partly be attributed to the attenuated expression of growth factors.
Authors: Erica Leonor Hofer; Vincent La Russa; Alba Elizabeth Honegger; Eduardo Oscar Bullorsky; Raúl Horacio Bordenave; Norma Alejandra Chasseing Journal: Stem Cells Dev Date: 2005-10 Impact factor: 3.272