Literature DB >> 25150992

Isolation of leukocytes from mouse central nervous system.

Iva Lelios1, Melanie Greter.   

Abstract

During neuroinflammatory or neurodegenerative diseases, it is often critical to characterize the composition of infiltrating immune cells. This protocol describes a reliable, fast, and simple method for the isolation of leukocytes from murine central nervous system (CNS) during steady state or inflammation for analysis by flow cytometry or other techniques.

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Year:  2014        PMID: 25150992     DOI: 10.1007/978-1-4939-1212-4_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  3 in total

1.  Direct vascular channels connect skull bone marrow and the brain surface enabling myeloid cell migration.

Authors:  Fanny Herisson; Vanessa Frodermann; Gabriel Courties; David Rohde; Yuan Sun; Katrien Vandoorne; Gregory R Wojtkiewicz; Gustavo Santos Masson; Claudio Vinegoni; Jiwon Kim; Dong-Eog Kim; Ralph Weissleder; Filip K Swirski; Michael A Moskowitz; Matthias Nahrendorf
Journal:  Nat Neurosci       Date:  2018-08-27       Impact factor: 24.884

2.  Pharmacological activation of the C5a receptor leads to stimulation of the β-adrenergic receptor and alleviates cognitive impairment in a murine model of familial Alzheimer's disease.

Authors:  Eleni Fella; Revekka Papacharalambous; Demos Kynigopoulos; Maria Ioannou; Rita Derua; Christiana Christodoulou; Myrto Stylianou; Christos Karaiskos; Alexia Kagiava; Gerasimou Petroula; Chryso Pierides; Maria Kyriakou; Laura Koumas; Paul Costeas; Elena Panayiotou
Journal:  Front Immunol       Date:  2022-08-26       Impact factor: 8.786

3.  Expression of Tmem119/Sall1 and Ccr2/CD69 in FACS-Sorted Microglia- and Monocyte/Macrophage-Enriched Cell Populations After Intracerebral Hemorrhage.

Authors:  Qian Li; Xi Lan; Xiaoning Han; Jian Wang
Journal:  Front Cell Neurosci       Date:  2019-01-09       Impact factor: 5.505

  3 in total

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