Wen-Yao Wang1, Hong-Fei Zhang2, Lei Wang2, Yan-Peng Ma2, Fei Gao2, Shao-Jun Zhang2, Li-Chao Wang2. 1. Department of General Surgery, The Second Hospital of Hebei Medical university, Shijiazhuang, 050000 Hebei, China. Electronic address: wangwenyaodoctor@126.com. 2. Department of General Surgery, The Second Hospital of Hebei Medical university, Shijiazhuang, 050000 Hebei, China.
Abstract
PURPOSE: The aim was to investigate the expression level of miR-21 in HCC tissues and its prognostic value among Asian population. METHODS: In the present study, expression of miR-21 was evaluated by qRT- PCR in tumor and adjacent non-neoplastic tissues in 119 HCC patients. The association of miR-21 expression with clinicopathological factors and the prognosis of HCC patients was also analyzed. Survival analysis was performed using the Kaplan-Meier method and Cox's proportional hazards model. RESULTS: We found that miR-21 expression was significantly higher in HCC tissues compared with normal adjacent liver tissues (P<0.0001). The 5-year overall survival (OS) of high miR-21 expression group was significantly shorter than that of low miR-21 expression group (40.2% vs. 70.7%; P=0.007). Moreover, the 5-year disease-free survival (DFS) of high miR-21 expression group was also significantly shorter than that of low miR-21 expression group (17.4% vs. 57.3%; P=0.001). Furthermore, in a multivariate Cox model, we found that miR-21 expression was an independent poor prognostic factor for both 5-year OS (hazards ratio [HR]=3.189, 95% confidence interval [CI]=1.911-10.012, P=0.03) and 5-year DFS (HR=5.897, CI=3.009-13.763, P<0.001) in HCC. CONCLUSIONS: The results of the present study suggested miR-21 expression level could be a novel potential biomarker for HCC prognosis.
PURPOSE: The aim was to investigate the expression level of miR-21 in HCC tissues and its prognostic value among Asian population. METHODS: In the present study, expression of miR-21 was evaluated by qRT- PCR in tumor and adjacent non-neoplastic tissues in 119 HCC patients. The association of miR-21 expression with clinicopathological factors and the prognosis of HCC patients was also analyzed. Survival analysis was performed using the Kaplan-Meier method and Cox's proportional hazards model. RESULTS: We found that miR-21 expression was significantly higher in HCC tissues compared with normal adjacent liver tissues (P<0.0001). The 5-year overall survival (OS) of high miR-21 expression group was significantly shorter than that of low miR-21 expression group (40.2% vs. 70.7%; P=0.007). Moreover, the 5-year disease-free survival (DFS) of high miR-21 expression group was also significantly shorter than that of low miR-21 expression group (17.4% vs. 57.3%; P=0.001). Furthermore, in a multivariate Cox model, we found that miR-21 expression was an independent poor prognostic factor for both 5-year OS (hazards ratio [HR]=3.189, 95% confidence interval [CI]=1.911-10.012, P=0.03) and 5-year DFS (HR=5.897, CI=3.009-13.763, P<0.001) in HCC. CONCLUSIONS: The results of the present study suggested miR-21 expression level could be a novel potential biomarker for HCC prognosis.
Authors: Joseph M Luna; Juan M Barajas; Kun-Yu Teng; Hui-Lung Sun; Michael J Moore; Charles M Rice; Robert B Darnell; Kalpana Ghoshal Journal: Mol Cell Date: 2017-07-20 Impact factor: 17.970
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Authors: Senwei Tang; William K K Wu; Xiangchun Li; Sunny H Wong; Nathalie Wong; Matthew T V Chan; Joseph J Y Sung; Jun Yu Journal: Sci Rep Date: 2016-04-15 Impact factor: 4.379