Karina Morais Faria1, Thais Bianca Brandão2, Ana Carolina Prado Ribeiro2, Adriele Ferreira Gouvêa Vasconcellos2, Icaro Thiago de Carvalho3, Fernando Freire de Arruda3, Gilberto Castro Junior4, Vanessa Cristina Gross1, Oslei Paes Almeida1, Marcio Ajudarte Lopes1, Alan Roger Santos-Silva5. 1. Oral Diagnosis Department, Semiology and Pathology Areas, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, Sao Paulo, Brazil. 2. Dental Oncology Service, Instituto do Câncer do Estado de São Paulo (ICESP), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. 3. Radiotherapy Service, Instituto do Câncer do Estado de São Paulo (ICESP), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. 4. Clinical Oncology Service, Instituto do Câncer do Estado de São Paulo (ICESP), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. 5. Oral Diagnosis Department, Semiology and Pathology Areas, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, Sao Paulo, Brazil. Electronic address: alanroger@fop.unicamp.br.
Abstract
INTRODUCTION: Teeth are often included in the radiation field during head and neck radiotherapy, and recent clinical evidence suggests that dental pulp is negatively affected by the direct effects of radiation, leading to impaired sensitivity of the dental pulp. Therefore, this study aimed to investigate the direct effects of radiation on the microvasculature, innervation, and extracellular matrix of the dental pulp of patients who have undergone head and neck radiotherapy. METHODS: Twenty-three samples of dental pulp from patients who finished head and neck radiotherapy were analyzed. Samples were histologically processed and stained with hematoxylin-eosin for morphologic evaluation of the microvasculature, innervation, and extracellular matrix. Subsequently, immunohistochemical analysis of proteins related to vascularization (CD34 and smooth muscle actin), innervation (S-100, NCAM/CD56, and neurofilament), and extracellular matrix (vimentin) of the dental pulp was performed. RESULTS: The morphologic study identified preservation of the microvasculature, nerve bundles, and components of the extracellular matrix in all studied samples. The immunohistochemical analysis confirmed the morphologic findings and showed a normal pattern of expression for the studied proteins in all samples. CONCLUSIONS: Direct effects of radiotherapy are not able to generate morphologic changes in the microvasculature, innervation, and extracellular matrix components of the dental pulp in head and neck cancer patients.
INTRODUCTION: Teeth are often included in the radiation field during head and neck radiotherapy, and recent clinical evidence suggests that dental pulp is negatively affected by the direct effects of radiation, leading to impaired sensitivity of the dental pulp. Therefore, this study aimed to investigate the direct effects of radiation on the microvasculature, innervation, and extracellular matrix of the dental pulp of patients who have undergone head and neck radiotherapy. METHODS: Twenty-three samples of dental pulp from patients who finished head and neck radiotherapy were analyzed. Samples were histologically processed and stained with hematoxylin-eosin for morphologic evaluation of the microvasculature, innervation, and extracellular matrix. Subsequently, immunohistochemical analysis of proteins related to vascularization (CD34 and smooth muscle actin), innervation (S-100, NCAM/CD56, and neurofilament), and extracellular matrix (vimentin) of the dental pulp was performed. RESULTS: The morphologic study identified preservation of the microvasculature, nerve bundles, and components of the extracellular matrix in all studied samples. The immunohistochemical analysis confirmed the morphologic findings and showed a normal pattern of expression for the studied proteins in all samples. CONCLUSIONS: Direct effects of radiotherapy are not able to generate morphologic changes in the microvasculature, innervation, and extracellular matrix components of the dental pulp in head and neck cancerpatients.
Authors: Rowida Abdalla; Maha A Niazy; Wael E Jamil; Hala A Hazzaa; Amal A Elbatouti Journal: Radiat Environ Biophys Date: 2017-03-23 Impact factor: 1.925
Authors: Caique Mariano Pedroso; Cesar Augusto Migliorati; Joel B Epstein; Ana Carolina Prado Ribeiro; Thaís Bianca Brandão; Márcio Ajudarte Lopes; Mário Fernando de Goes; Alan Roger Santos-Silva Journal: Front Oral Health Date: 2022-07-14
Authors: K Morais-Faria; R Neves-Silva; M-A Lopes; A-C-P Ribeiro; G de Castro; K-G-M da Conceição-Vasconcelos; T-B Brandão; A-R Santos-Silva Journal: Med Oral Patol Oral Cir Bucal Date: 2016-05-01