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Literature DB >> 25143570

Updating the PGMY primers and probes for improved detection of HPV68a: validation of version 2 of the PGMY-CHUV assay.

Abstract

HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific for the other HPV genotypes during a 1-year prospective validation (n = 714 samples).

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Year: 2014 PMID： 25143570 PMCID： PMC4313245 DOI： 10.1128/JCM.01950-14

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

12 in total

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Review 2. Chapter 1: HPV in the etiology of human cancer.

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4. Validation of a low-cost human papillomavirus genotyping assay based on PGMY PCR and reverse blotting hybridization with reusable membranes.

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Journal: J Clin Microbiol Date: 2011-08-10 Impact factor: 5.948

5. Comparison of Seegene Anyplex II HPV28 with the PGMY-CHUV assay for human papillomavirus genotyping.

Authors: C Estrade; R Sahli
Journal: J Clin Microbiol Date: 2013-12-11 Impact factor: 5.948

6. Genotyping of 27 human papillomavirus types by using L1 consensus PCR products by a single-hybridization, reverse line blot detection method.

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7. Human papillomavirus type distribution in invasive cervical cancer and high-grade cervical lesions: a meta-analysis update.

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Review 8. Chapter 3: HPV type-distribution in women with and without cervical neoplastic diseases.

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9. Global proficiency study of human papillomavirus genotyping.

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Review 10. Papillomaviruses in the causation of human cancers - a brief historical account.

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4. Impact of HPV vaccination with Gardasil® in Switzerland.

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4 in total