| Literature DB >> 25139233 |
Shirou Tsuchida1, Mao Tamura2, Naoya Hamaue2, Takashi Aoki2.
Abstract
A novel cloning vector that can be used to identify recombinant Escherichia coli colonies by activation of the green fluorescent protein gene (GFP) was constructed. Screening using the vector does not require special reagents. The recombinant plasmid activates GFP, and the rate of false-positive results is low.Entities:
Keywords: Cloning vector; Escherichia coli; Green fluorescent protein
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Year: 2014 PMID： 25139233 DOI： 10.1016/j.bbrc.2014.08.038
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575