Contribution of neuraminidase of influenza viruses to the sensitivity to sera inhibitors and reassortment efficiency.

Live attenuated influenza vaccine (LAIV) represent reassortant viruses with hemagglutinin (HA) and neuraminidase (NA) gene segments inherited from circulating wild-type (WT) parental influenza viruses recommended for inclusion into seasonal vaccine formulation, and the 6 internal protein-encoding gene segments from cold-adapted attenuated master donor viruses (genome composition 6:2). In this study, we describe the obstacles in developing LAIV strains while taking into account the phenotypic peculiarities of WT viruses used for reassortment. Genomic composition analysis of 849 seasonal LAIV reassortants revealed that over 80% of reassortants based on inhibitor-resistant WT viruses inherited WT NA, compared to 26% of LAIV reassortants based on inhibitor-sensitive WT viruses. In addition, the highest percentage of LAIV genotype reassortants was achieved when WT parental viruses were resistant to non-specific serum inhibitors. We demonstrate that NA may play a role in influenza virus sensitivity to non-specific serum inhibitors. Replacing NA of inhibitor-sensitive WT virus with the NA of inhibitor-resistant master donor virus significantly decreased the sensitivity of the resulting reassortant virus to serum heat-stable inhibitors.

INTRODUCTION

The Russian live attenuated influenza vaccine (LAIV) consists of reassortant viruses, which contain hemagglutinin (HA) and neuraminidase (NA) gene segments from circula-ting wild-type (WT) viruses which are recommended on annual basis for inclusion in a seasonal vaccine formulation on a background of internal genes derived from the attenuated vaccine master donor viruses (MDVs), A/Leningrad/134/17/57 (H2N2) or B/USSR/60/69 (representing genomic composition of 6 MDV genes and 2 WT genes). LAIV strains possess the cold-adapted, temperature-sensitive and attenuated phenotypes from the MDVs. According to the Russian regulatory practice, 5:3 genomic composition is also acceptable for LAIV [1] if the third WT gene of the vaccine candidate does not encode RNA polymerase protein complex components.

Based on ongoing world-wide surveillance of influenza virus circulation, the World Health Organization (WHO) annually recommends viruses that antigenically match to circulating strains in order to be included in influenza vaccines [2]. As the time for vaccine virus generation is extremely limited, and because only 6:2 and 5:3 reassortant

^These authors contributed equally to this work. virus ratios are allowed as vaccine candidates, the methods for vaccine development must be rapid, reproducible, and reliable. The most successful and well-established current strategy for preparing attenuated reassortant vaccine virus has been to co-infect embryonated chicken eggs with equal doses of selected WT virus with cold-adapted (CA) MDV. Co-infection with MDV and WT viruses theoretically may yield as many as 256 combinations of gene segments upon gene reassortment, and, thus, 256 genetically different genotypes. To diminish the number of undesired reassortant viruses arising during co-infection, two selecting factors - serum raised against the MDV and low incubation temperature (25-26 °C) - are used in subsequent selective passages [3, 4].

Successful and quick generation of LAIV reassortants depends considerably on a variety of factors, including the phenotypic properties of the WT parental strain. Recently, many strains identified by the WHO as those likely to resemble the strains in circulation during the next epidemic season have been found to be highly sensitive to non-specific serum inhibitors [5, 6]. Sera and other biological liquids of different animal species contain non-specific inhibitors of influenza viruses. Composition and concentration of inhibitors depends on animal species [7, 8]. Mechanism of interaction of different inhibitor classes with influenza virus is described in set of studies [7- 9]. The standard protocol for developing LAIV reassortants includes treating anti-MDV serum with receptor destroying enzyme (RDE) to remove non-specific inhibitors. However, properly treated serum may still contain residual levels of heat-stable inhibitors. The presence of residual non-specific inhibitors in immune anti-MDV serum may alter the antibody recognition pattern and complicate development of reassortant vaccine strains based on inhibitor-sensitive WT viruses. Additionally, some early publications debated whether the LAIV candidate should be inhibitor-sensitive or resistant. Some researchers believed that serum inhibitor-resistant LAIV candidates are more attenuated and, therefore, more attractive for human use [10].

In this paper, we analyzed 849 reassortants of several WT viruses with MDVs obtained using classical egg-based reassortment techniques. We describe some aspects of developing LAIV reassortants, including obstacles in development, importance of WT parental virus properties, and differences in how seasonal human influenza viruses and H5N1 avian influenza viruses reassort with MDVs.

MATERIALS AND METHODOLOGY

Viruses

A total of 44 influenza A and B viruses were chosen for this study. Of these, 37 WT influenza A and B viruses were obtained from the Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA). Reassortant vaccine candidates for parenterally administered, inactivated influenza vaccine of subtypes H5N1 (NIBRG-23, INDO/05, VN1203), H3N2 (NIB-64), and H1N1 (IRV-148) based on A/PR/8/34 (H1N1) strain (PR8) as the high-yield internal gene donor were generated by reverse genetics techniques from A/turkey/Turkey/1/05 (H5N1), A/Indonesia/5/05 (H5N1), A/Vietnam/1203/2004 (H5N1), A/Perth/16/09 (H3N2), and A/Brisbane/59/07 (H1N1) influenza viruses, respectively. The cleavage site of genes coding HA of H5N1 WT viruses was modified by removing 4 basic amino acid codons. PR8-based reassortants were obtained from CDC and WHO. Two cold-adapted MDVs, A/Leningrad/134/ 17/57 (H2N2) (A-MDV) and B/USSR/60/69 (B-MDV), that are used to develop the Russian LAIV are the property of the Institute of Experimental Medicine (St. Petersburg, Russia).

Viruses were propagated in 10-11-day-old embryonated chicken eggs at 32-33 °C.

Genetic Reassortment Procedures

MDV and WT viruses were co-infected into embryonated chicken eggs by classical reassortment techniques as described previously [3]. Briefly, 5-6 rounds of selective propagation were performed, 3 of them at 25-26 °C. The production and selection of reassortants were carried out in the presence of rabbit or rat RDE-treated MDV antiserum. Cloning by endpoint dilution was performed in each of the last 3-4 passages.

RNA Isolation

RNA was isolated from influenza virus-infected allantoic fluid using a QIAamp Viral RNA minikit per manufacturer’s instructions (Qiagen GmbH, Hilden, Germany).

Genomic Composition Analysis

The parental origin of the RNA segments of each reassortant virus was monitored by restriction fragment length polymorphism (RFLP) analysis [11], or determined by mixed PCR assays [12]. Nucleotide sequencing was performed using a BigDye Terminator v3.1 cycle sequencing kit and a 3130xl Genetic Analyzer (both from Applied Biosystems, Carlsbad, CA, USA) according to manufacturer’s instructions. Multiple sequence alignment analysis was performed using Lasergene version 7.1 sequence analysis software.

Hemagglutination Inhibition (HAI) Assays

HAI assays were performed using standard techniques as described in [13], in 96-well microtiter plates at room temperature with 1.0% suspension of chicken or human 0(I) Rh+ red blood cells.

Sensitivity to Non-Specific Serum Inhibitors

To analyze the sensitivity of influenza viruses to non-specific inhibitors, normal guinea pig serum (Rappolovo Laboratory Animals Farm, St. Petersburg, Russia) was used. Serum was heat-inactivated for 10 min at 80 °C to eliminate temperature-sensitive inhibitors, and used for HAI assays with 4 hemagglutinating units of each viruses. A virus was considered to be resistant to non-specific serum inhibitors if the HAI titer was ≤ 40, and designated as sensitive to non-specific serum inhibitors if HAI titer was ≥ 80.

Statistical Analyses

Pearson chi-square (χ2) test was used to identify associations between inhibitor sensitivity of parental viruses used for reassortment and reassortment efficiency. Student's t distribution test for dependent samples was used to evaluate the differences in geometrical mean HAI titers between groups of viruses compared for their ability to react with normal guinea pig sera. A value of p < 0.001 was considered significant, and p < 0.0001 was considered highly significant.

RESULTS

Reassortment of Seasonal WT Influenza Viruses with MDVs for LAIV Generation

Most influenza A and B viruses circulating prior to the year 2000 readily reassorted with MDVs, and reassortants with the desired vaccine genomic composition of 6:2 and 5:3 were easy to generate. The resulting reassortants typically inherited 60-90% of internal genes from MDVs, and 90-100% HA and NA genes from WT parents. In contrast, vaccine strains based on WT viruses circulating after year 2000 were much more difficult to develop. In our experience only 11-14% of reassortants derived from post-2000 viruses using standard techniques possessed NA of WT parental virus. The percentage of internal genes derived from MDVs, especially M and NS, was also relatively low. WT- and MDV-gene segment distribution in the genome of reassortants generated on the basis of some influenza A and B viruses circulating in various years is presented in Table .

The difficulties encountered in generating current LAIV reassortant viruses led us to perform a large-scale analysis of reassortment efficiency. We found that the rate of NA inheritance by reassortant viruses depended on the inhibitor sensitivity of the WT parental virus to normal guinea pig serum regardless of its specific type/subtype, with the exception of subtype H5N1 viruses. Table shows the distribution of reassortants bearing NA gene segments from WT or cold-adapted parental viruses stratified by the inhibitor sensitivity of WT parental viruses. In fact, over 80% of all reassortants based on inhibitor-resistant WT viruses (297 out of 356) inherited WT NA, compared to only ~26% of reassortants based on inhibitor-sensitive WT viruses (130 out of 493; Fig. ). The rate of WT NA inheritance by influenza virus reassortants of various types/subtypes varied, but generally was significantly higher when parental viruses were inhibitor-resistant (Table ).

Genomic analysis of 849 reassortants revealed that the highest percentage of 6:2 and 5:3 vaccine-appropriate genotype reassortants was achieved when using WT parental viruses resistant to non-specific serum inhibitors (Fig. ). When using inhibitor-resistant group of influenza viruses, vaccine-appropriate reassortants (with either 6:2 and 5:3 gene composition) were found in the 108/136 (79%; H1N1 subtype), 5/5 (100%; H2N2 subtype), 32/74 (43%; H3N2 subtype), and 51/88 (58%; type B) cases. In contrast, when using inhibitor-sensitive WT parental viruses, only 4/29 (14%; H2N2 subtype), 25/126 (20%; H3N2 subtype), and 24/326 (7%; type B) vaccine reassortants were isolated (Table ). Since only 6:2 and 5:3 genomic combinations are appropriate for generating LAIV strains, we correlated the number of reassortants with vaccine genomic composition and all other genomic combinations. Collected data were analyzed by Pearson χ2 test (Table ). Reassortants with 6:2 or 5:3 genomic composition were generated significantly more frequently from inhibitor-resistant WT influenza viruses (χ2 = 195.78; p < 0.0001).

Role of NA in Inhibitor Sensitivity of Reassortant Viruses

We analyzed the following viruses for their reactivity to normal guinea pig serum: LAIV MDVs A/Leningrad/134/ 17/57 (H2N2) and B/USSR/60/69; four influenza A WT viruses; six influenza B WT viruses; six 6:2 reassortants containing HA and NA from WT virus and internal genes from MDV (R2, R10, R13, R15, R16, R19); and thirteen 7:1 reassortants with a single HA gene segment from the WT influenza virus and 7 RNA segments from MDV (R1, R3-R9, R11, R12, R14, R17, R18). In the HAI assay, 6 out of 9 WT parental viruses chosen for this study were highly sensitive to inhibition by normal guinea pig serum (Table ). The 2 MDVs, A/Leningrad/134/17/57 and B/USSR/60/69, and WT viruses VN1203, INDO/05, and B/Texas/26/08, were highly resistant to inhibitors, as HAI titers did not exceed 10.

The degree of sensitivity (HAI titer) to serum differed dramatically among the 6:2 and 7:1 reassortants generated on the basis on inhibitor-sensitive WT viruses. All 7:1 reassortants possessing HA from inhibitor-sensitive and NA from inhibitor-resistant MDVs were significantly less sensitive to inhibitors than their WT parental viruses or 6:2 reassortants (t = 11.09; p < 0.00001; Table ). While still inhibitor-sensitive, the 7:1 reassortants displayed a ≥ 4-fold drop in HAI titer with normal guinea pig serum compared to viruses possessing HA and NA from inhibitor-sensitive WT parental virus. For instance, the HAI titer of NIBRG-23 (H5N1) virus, which is highly sensitive to normal guinea pig serum, was 5120. In contrast, the HAI titer of the R3 7:1 reassortant was only 640 when using the same serum.

To determine whether the observed change in inhibitor sensitivity of 7:1 reassortants resulted directly from altered NA function, we compared the sequences of parental viruses and their single gene reassortants. Interestingly, we found identical mutations in the MDV NA gene of several reassortants within type A (nt 765 in R3 and R9) and type B (nt 1085 and nt 1359 in R11 and R17; in R14 and R18) viruses (Table ). These mutations could be new spontaneous mutations in positions responsible for the adaptation of the virus to chicken eggs, or a minor quasispecies in the initial viral stock. In either case, these mutations did not affect the phenotype of R4 virus, and none of the mutations altered the viruses’ sensitivity to heat-stable inhibitors. For instance, R3 was exactly as sensitive as the 2 other NIBRG-23 × A-MDV reassortants, R4 and R5.

Thus, the change in inhibitor sensitivity of 7:1 reassortants is likely to result from alterations in NA function rather than mutations.

Reassortment of H5N1 Influenza Viruses with A-MDV for LAIV

Highly pathogenic avian influenza viruses, having already overcome the species barrier in a number of cases, pose a potential pandemic threat. H5N1/PR8 candidates for inactivated influenza vaccines based upon viruses with pandemic potential are generated by reverse genetics methods by removing the multibasic amino acid motif associated with high pathogenicity in the HA gene and combining surface glycoprotein genes from avian virus with genes for internal proteins from A/Puerto Rico/8/34 (PR8) backbone. Recently, attempts have been made to use these attenuated H5N1/PR8 viruses as the source of HA and NA for classical reassortment with MDV to generate LAIV. However, mating A-MDV with either VN1203 or INDO/05 H5N1/PR8-based viruses has resulted in reassortants containing a single HA gene segment of the H5N1 influenza virus, and 7 RNA segments, including NA, from the A-MDV. Another H5N1/PR8 virus, NIBRG-23, did not reassort with A-MDV at all.

In an attempt to disrupt the incredibly strong gene constellation of NIBRG-23, this virus was inactivated with ultraviolet light to lower the infectious titer by 6 log10 effective infection dose 50 (EID50)/mL as previously described [15]. The inactivated virus was then used for co-infection with A-MDV in attempt of classical reassortment. One triple reassortant virus was generated in the initial co-infection, containing PB2, NP, and NS genes from PR8 virus, PB1, PA, and M genes from A-MDV, and HA and NA genes from H5N1. We then back-crossed this progeny virus with A-MDV in order to obtain the desired 6:2 reassortant virus. However, we were unable to generate 6:2 H5N1 LAIV candidates: we isolated 3 cold-adapted and temperature sensitive reassortants from the back-cross, but they were of the 7:1 genotype. Thus, we did not detect any 6:2 reassortants with Leningrad backbone when using H5N1/PR8 viruses as the source of HA and NA.

Interestingly, we found a strong interaction between genes encoding avian NA and PB2 gene of PR8 virus in the genomes of H5N1/PR8 viruses. On the contrary, we also saw a balance between PB2/MDV and NA/MDV. Reassortants co-inherited the PB2/PR8 gene with NA/H5N1 gene, and PB2/MDV gene with NA/MDV gene (Table ). We thus began a new series of experiments to determine the factors influencing co-inheritance of PB2 and NA genes of H5N1/PR8 reassortants. Table shows the results of co-infecting A-MDV with PR8-based reassortant vaccine candidates for inactivated seasonal influenza vaccine of H3N2 (NIB-64) or H1N1 (IRV-148) subtypes. We isolated 28 reassortants based on IRV-148 (H1N1), and 9 reassortants based on NIB-64 (H3N2). This experiment indicated that all reassortants received WT NA. Additionally, 5/9 (55%) NIB-64-based reassortants and all 28 IRV-148-based reassortants inherited A-MDV PB2 gene. Moreover, 6 IRV-148-based reassortants and 2 NIB-64-based reassortants were 6:2.

DISCUSSION

Vaccines are considered the best available protection against influenza. Live attenuated influenza vaccines contain reassortant viruses expressing HA and NA gene segments from circulating WT viruses, and the internal genes from cold-adapted attenuated MDVs. A widely used classical method of making reassortant vaccine virus is based on co-infecting a WT strain and a cold-adapted MDV into embryonated chicken eggs. Theoretically, 256 potential genotypes could arise from the reassortment between 2 influenza viruses. To increase the number of reassortants possessing a vaccine-appropriate genomic composition, 2 selecting factors, anti-MDV serum and low incubating temperatures (25-26 °C), are used [3, 4].

LAIV strain development and characterization takes at least 2.5-3 months, and is a reasonably complicated and difficult process. Successful and fast generation and characterization of vaccine reassortants depends on numerous factors. In this study we show that most influenza A and B viruses that circulated in the 1990s readily reassorted with MDVs, and reassortants with the desired vaccine genotype were easy to generate. The majority of the resulting reassortants inherited all internal genes from MDVs and HA and NA from WT parental viruses. In contrast, vaccine strains based on most recently circulating viruses were much more difficult to develop. Only 11-14% of reassortants possessed NA from the WT parental virus, and the percentage of internal genes derived from MDVs was relatively low.

Analysis of 849 reassortants based on influenza viruses circulating in different years revealed that clones with an expected 6:2 vaccine genotype were isolated much more frequently when using WT parental viruses resistant to non-specific serum inhibitors. Thus, over 80% of the reassortants based on inhibitor-resistant WT viruses inherited WT NA. In contrast, only 26% of the reassortants based on inhibitor-sensitive WT viruses inherited WT NA. Using inhibitor-resistant variants obtained from inhibitor-sensitive WT viruses recommended by WHO for influenza vaccines may help to solve the problem of reliably obtaining 6:2 reassortants.

Based on these findings, we wanted to determine the role of NA in the efficiency of influenza virus reassortment, and why inhibitor-sensitive WT viruses so rarely donate NA to reassortants. The receptor-binding activity of the influenza virus can be non-specifically inhibited by inhibitors present in normal sera, though the molecular mechanisms of serum resistance in influenza viruses are not well known. Previous studies demonstrated that HA plays an important role in inhibitor sensitivity of influenza H3N2 virus [8]. However, these data rely on the assumption that reassortant viruses inherit an inhibitor-sensitive phenotype with the HA of the inhibitor-sensitive parental virus. Gimsa et al. [9] demonstrated that NA may also play a role in inhibitor sensitivity.

In the present study, we investigated the sensitivity of single-gene reassortants to non-specific inhibitors, and compared this sensitivity to that of their parental viruses. We introduced HA into MDV genomes following a reassortment event with inhibitor-sensitive or inhibitor-resistant WT influenza viruses of subtypes H3N2 and H5N1, and of type B. Replacing the NA of an inhibitor-sensitive WT virus with the NA of an inhibitor-resistant MDV (reassortants R1, R3-R5, R11, R14, R17, R18) led to significantly reduced serum inhibitor sensitivity of the resulting reassortant virus. These data indicate that NA plays a role in the sensitivity of influenza viruses to non-specific serum inhibitors. Thus, viral sensitivity to inhibitors seems to depend on a combination of unique properties of HA and NA.

Many strains recently identified by the WHO for inclusion into seasonal influenza vaccines, including most influenza B viruses of the Yamagata lineage, are highly sensitive to non-specific serum inhibitors [5, 6]. The standard protocol of developing reassortants LAIVs contains a step of treating anti-MDV serum with RDE to remove non-specific inhibitors. However, even when anti-MDV serum was appropriately treated with RDE, it may still contain a residual amount of NA inhibitors [7, 16, 17], which could influence reassortment if the WT parental virus is inhibitor-sensitive. Passaging reassortant progeny in the presence of anti-MDV serum is another step in the LAIV preparation protocol [3, 4]; thus, reassortants possessing the desired HA and NA of the inhibitor-sensitive WT parent might be non-specifically suppressed because of residual anti-NA activity in the serum.

Earlier publications discussed whether LAIV reassortant candidates should be inhibitor-sensitive or resistant. Serum inhibitor-resistant LAIV candidates were thought to be more suitable for human use [10]. However, naturally occurring inhibitors of influenza virus HA activity have been identified both in various sera (including human sera) and in fluid secretions [7, 18-20]. Respiratory tract mucosa is not only the site of viral infection, but also the site of defense against infection. From this point of view, inhibitor-resistant vaccine viruses are more likely to bind to and enter the sensitive host cells than those derived from inhibitor-sensitive WT viruses, and may be better suited for use in humans.

The H5N1 avian influenza viruses are highly pathogenic and have already overcome the species barrier, causing justifiably serious concern of a new pandemic. A novel approach to developing potential pandemic LAIV has been described recently. The central idea was to develop LAIV candidates by using H5N1/PR8 viruses attenuated by reverse genetics as the source of HA and NA. However, the resulting reassortants possessed H5 HA, while the 7 other genes came from A-MDV [21]. Neither were we successful in generating 6:2 or 5:3 H5N1 LAIV candidates by classical reassortment with the A-MDV in this study. Our study identifies influenza virus gene segments that appear to be incompatible for generating H5N1/PR8-MDV reassortants. For instance, we found a strong link between genomic segments encoding avian origin NA and PR8 virus PB2 gene. We also observed a functional balance between H5N1 HA, MDV NA, and MDV PB2. This strong constellation effect between gene segments of parental viruses may affect reassortment.

A little is known about an efficacy of reassortment in vitro or in vivo. Some pairs of viruses are difficult to reassort, as seen in attempts to generate avian-human reassortant viruses with the desired genomic composition. Subarrao et al. [22] obtained 6:2 reassortants of H3N2 human virus with gull influenza A virus, but the attempt to generate 6:2 reassortants of H1N1 human viruses with the same gull influenza A virus was not successful. The authors suggested that some influenza virus gene segments appear to be incompatible for generation of reassortants.

Indeed, reassortment, especially obtaining reassortants with desired gene combinations (6:2, for instance) is really rare event. In recent publication [23] interesting observation was made that pandemic “H1N1 virus could not be reproduced either in cell cultures or in pigs coinfected with North American triple reassortant H1N1 and Eurasian H1N1 swine influenza viruses.”

Molecular basis of such restriction is still unknown. We can only speculate that the nature of this process depends on combination of as yet unknown factors.

In our experiments, NIBRG-23 (H5N1) virus did not reassort with A-MDV at all. Only inactivating NIBRG-23 by ultraviolet light prior to reassortment allowed generation of 7:1 H5N2 reassortants. INDO/05 and VN1203 did reassort with the resulting 7:1 reassortant progeny without inactivating the WT parental virus. It is difficult to fully understand the reasons for the difficulties in reassorting some H5N1 avian viruses with the A-MDV (H2N2) human strain.

The experimental inability to generate 6:2 H5N1 LAIV candidates by classical reassortment should not discourage the use of a 7:1 H5N2 potential pandemic vaccine in case of need. The presence of NA from cold-adapted MDV, together with genetically modified HA/H5N1, should provide additional attenuation of H5N2 LAIV candidate against the highly pathogenic H5N1 avian influenza virus. Our previous study of MDV-specific mutations revealed that reassortant viruses containing HA and NA genes from A-MDV, and the 6 other genes from its WT progenitor, were significantly more temperature-sensitive than WT virus itself (I. Isakova-Sivak, personal communication). This finding indicates that the A-MDV NA gene alone or together with the HA gene can potentially contribute to the attenuated phenotype of the MDV. Therefore, including the MDV NA gene into a H5 vaccine strain may improve vaccine safety. Furthermore, according to the generally accepted concept, the anti-HA antibody response is the main contributor in protection of humans against influenza. The anti-NA antibody can modify the severity of the illness, but is not as crucial as anti-HA antibodies. Avian influenza viruses are not an exception. Immunizing chickens with HA alone or in combination with NA induced complete protection against challenge with the H5N1 avian influenza virus [24]. Thus, 7:1 H5N2 reassortants are promising candidates for further evaluation in humans.

CONCLUSION

In conclusion, our data suggest that NA plays an important role in the efficiency of reassortment of WT parental viruses with CA MDVs. We also demonstrated that NA contributes to the inhibitor-sensitive phenotype of influenza reassortant viruses and affects reassortment efficiency. Clearly, LAIV development would benefit from a better understanding of the genetic features and mechanisms of influenza virus serum resistance. Such studies must continue and expand to identify the mechanisms of serum sensitivity and gene constellations in the genome of H5N1/PR8 reassortants.

Table 1.

Gene segment distribution in the genomes of reassortants generated from various influenza A and B viruses circulating in different years.

Influenza A Viruses
Gene Segments	Before 20001				After 20002
	%	(n)	%	(n)	%	(n)	%	(n)
	WT		MDV		WT		MDV
PB2	2,1%	(2)	97,9%	(95)	9,6%	(5)	90,4%	(47)
PB1	5,2%	(5)	94,8%	(92)	40,4%	(21)	59,6%	(31)
PA	2,1%	(2)	97,9%	(95)	9,6%	(5)	90,4%	(47)
HA	100,0%	(97)	0,0%	(0)	100,0%	(52)	0,0%	(0)
NP	29,9%	(29)	70,1%	(68)	44,2%	(23)	55,8%	(29)
NA	91,8%	(89)	8,2%	(8)	13,5%	(7)	86,5%	(45)
M	42,3%	(41)	57,7%	(56)	65,4%	(34)	34,6%	(18)
NS	47,4%	(46)	52,6%	(51)	80,8%	(42)	19,2%	(10)
Influenza B Viruses
Gene Segments	Before 20003				After 20004
	%	(n)	%	(n)	%	(n)	%	(n)
	WT		MDV		WT		MDV
PB2	8,3%	(1)	91,7%	(11)	19,9%	(48)	80,1%	(193)
PB1	17,0%	(2)	83,0%	(10)	39,8%	(96)	60,2%	(145)
PA	16,7%	(2)	83,3%	(10)	10,0%	(24)	90,0%	(217)
HA	100,0%	(12)	0,0%	(0)	100,0%	(241)	0,0%	(0)
NP	41,7%	(5)	58,3%	(7)	39,8%	(96)	60,2%	(145)
NA	91,7%	(11)	8,3%	(1)	11,2%	(27)	88,8%	(214)
M	50,0%	(6)	50,0%	(6)	90,0%	(217)	10,0%	(24)
NS	66,7%	(8)	33,3%	(4)	78,0%	(188)	22,0%	(53)

Influenza A viruses:

1Reassortants of A/Leningrad/134/17/57 (H2N2) master donor virus (MDV) with A/Nanchang/933/95 (H3N2), A/Beijing/262/95 (H1N1), A/Perth/13/95 (H1N1), A/Johannesburg/82/96 (H1N1), A/Panama/2007/99 (H3N2), A/New Caledonia/20/99 (H1N1)

2Reassortants of A/Leningrad/134/17/57 (H2N2) MDV with A/Wellington/01/04 (H3N2), A/Malaysia/01/04 (H3N2), and A/Brisbane/10/07 (H3N2).

Influenza B viruses:

3Reassortants of B/USSR/60/69 MDV with B/Harbin/07/94, B/Shandong/7/97

4Reassortants of B/USSR/60/69 MDV with B/Florida/4/06, B/Brisbane/3/07, B/Bangladesh/1994/10, and B/Wisconsin/1/10

Table 2.

Rate of neuraminidase (NA) gene segment inheritance by reassortants of master donor viruses (MDV) with wild-type (WT) influenza viruses of varying sensitivity to non-specific, heat-stable inhibitors in guinea pig serum.

WT Parental Strain1		Reassortants Generated2
Strain Designation	Sensitivity to Inhibitors	Total	NA Gene From
			WT Parent	MDV
Influenza A (H3N2) Viruses
A/ Hong Kong/1186/03	Resistant	8	8	0
A/Wyoming/3/03	Resistant	4	4	0
A/California/07/04	Resistant	27	26	1
A/Wisconsin/67/05	Resistant	19	17	2
A/Victoria/361/11	Resistant	16	16	0
A/Nanchang/933/95	Sensitive	9	9	0
A/Sydney/5/97	Sensitive	58	2	56
A/Panama/2007/99	Sensitive	7	1	6
A/Wellington/01/04	Sensitive	30	9	21
A/Malaysia/01/04	Sensitive	8	2	6
A/Brisbane/10/07	Sensitive	14	3	11
Influenza A (H1N1) Viruses
A/Beijing/262/95	Resistant	18	15	3
A/Perth/13/95	Resistant	7	7	0
A/Johannesburg/82/96	Resistant	6	6	0
A/Brisbane/59/07	Resistant	13	11	2
A/New Caledonia/20/99	Resistant	50	50	0
A/Solomon Islands/03/06	Resistant	8	8	0
A/California/07/09	Resistant	34	33	1
Influenza A (H2N2) Viruses
A/ California/1/66	Resistant	5	5	0
A/Tokyo/3/67	Sensitive	29	29	0
Influenza A (H5N1) Viruses
NIBRG-23	Sensitive	123	12	0
VN1203	Resistant	42	14	28
INDO/05	Resistant	11	0	11
Influenza B Viruses
B/Shandong/7/97	Resistant	8	8	0
B/Hong Kong/330/01	Resistant	6	6	0
B/Malaysia/2506/04	Resistant	29	27	2
B/Ohio/01/05	Resistant	17	17	0
B/Brisbane/60/08	Resistant	16	14	2
Influenza B Viruses
B/Texas/26/08	Resistant	12	5	7
B/Harbin/07/94	Sensitive	4	3	1
B/Petersburg/92/95	Sensitive	8	3	5
B/Johannesburg/05/99	Sensitive	14	5	9
B/Jilin/20/03	Sensitive	10	4	6
B/Jangsu/10/03	Sensitive	23	9	14
B/Florida/7/04	Sensitive	14	5	9
B/Florida/4/06	Sensitive	31	3	28
B/Brisbane/3/07	Sensitive	13	1	12
B/Wisconsin/1/10	Sensitive	140	4	136
B/Bangladesh/1994/10	Sensitive	57	19	38
B/Texas/6/11	Sensitive	12	7	5

1WT parental virus. 2All reassortants inherited HA of WT viruses. 3Virus NIBRG-23 (H5N1) did not reassort with MDV when classical reassortment procedures were used; all 12 clones isolated were identical to NIBRG-23 (H5N1) in genomic composition.

Table 3.

Genomic composition and rate of NA inheritance by reassortants of MDV and currently circulating influenza viruses of varying sensitivity to non-specific, heat-stable inhibitors in guinea pig serum.

WT Parental Strain1		Reassortants Generated2
Number of Viruses Tested	Sensitivity to Inhibitors	Total Number	NA From		Genomic Composition3
			WT (%)	MDV (%)	6:2 (%)	5:3 (%)	7:1 (%)	Other (%)
Influenza A (H3N2) Viruses
5	Resistant	74	71 (95.9%)	3 (4.1%)	7 (9.5%)	25 (33.8%)	1 (1.4%)	41 (55.3%)
6	Sensitive	126	26 (20.6%)	100 (79.4%)	12 (9.5%)	13 (10.3%)	10 (7.9%)	91 (72.2%)
Influenza A (H1N1)4 Viruses
7	Resistant	136	130 (95.6%)	6 (4.4%)	71 (52.2%)	37 (27.2%)	0	28 (20.6%)
Influenza A (H2N2) Viruses
1	Resistant	5	5 (100%)	0	4 (80.0%)	1 (20.0%)	0	0
1	Sensitive	29	29 (100%)	0	0	4 (13.8%)	0	25 (86.2%)
Influenza A (H5N1) Viruses
2	Resistant	53	14 (26.4%)	39 (73.6%)	0	0	39 (73.6%)	14 (26.4%)
1	Sensitive	12	12 (100%)	0	0	0	0	12 (100%)
All Influenza A Viruses
14	Resistant	268	220 (82.1%)	48 (17.9%)	82 (30.6%)	63 (23.5%)	40 (14.9%)	83 (31.0%)
9	Sensitive	167	67 (40.1%)	100 (59.9%)	12 (7.2%)	17 (10.2%)	10 (6%)	128 (76.6%)
All Influenza B Viruses
6	Resistant	88	77 (87.5%)	11 (12.5%)	21 (23.9%)	30 (34.1%)	1 (1.1%)	36 (40.9%)
11	Sensitive	326	63 (19.3%)	263 (80.7%)	12 (3.7%)	12 (3.7%)	91 (27.9%)	211 (64.7%)

1The complete list of WT parental viruses is presented in Table 1. 2All reassortants inherited HA from WT parental viruses. 3Genomic composition: 6:2 are reassortants containing HA and NA from WT virus; 5:3 are reassortants containing HA and NA, from the WT parent, as well as WT NP, M, or NS gene; 7:1 are single gene reassortants containing only HA from WT virus. 4No inhibitor-sensitive WT viruses were found among the type A (H1N1) influenza viruses tested.

Table 4.

Statistical analyses of collected data using Pearson chi-square (χ2) test and Student’s t distribution test.

Virus Tested	Sensitivity to Inhibitors1	Comparison Groups	Number of Reassortants/Total	Pearson c2 Criterion	p Value
Efficiency of NA Inheritance by Reassortant Viruses
All A viruses	Resistant	WT-NA	220/268	80.74	p < 0.0001
	Sensitive		67/167
	Resistant	MDV-NA	48/268
	Sensitive		100/167
All B viruses	Resistant	WT-NA	77/88	143.90	p < 0.0001
	Sensitive		63/326
	Resistant	MDV-NA	11/88
	Sensitive		263/326
All A and B viruses	Resistant	WT-NA	297/356	269.21	p < 0.0001
	Sensitive		130/493
	Resistant	MDV-NA	59/356
	Sensitive		363/493
Efficiency of Reassorting Into Vaccine-appropriate (6:2 and 5:3) Genomic Compositions
All A viruses	Resistant	Vaccine genomic composition	145/268	57.86	p < 0.0001
	Sensitive		29/167
	Resistant	Other combinations	123/268
	Sensitive		138/167
All B viruses	Resistant	Vaccine genomic composition	51/88	119.57	p < 0.0001
	Sensitive		24/326
	Resistant	Other combinations	37/88
	Sensitive		302/326
All A and B viruses	Resistant	Vaccine genomic composition	196/356	195.78	p < 0.0001
	Sensitive		53/493
	Resistant	Other combinations	160/356
	Sensitive		440/493

Comparison Groups		t Distribution	p Value
Significance of Decreased Inhibitor Sensitivity in Reassortant Viruses (HAI titer)
WT viruses possessing inhibitor-resistant HA2 and inhibitor-resistant NA2 (HAI4 < 10)	6:2 WT/MDV reassortants possessing inhibitor-resistant HA2 and inhibitor-resistant NA2 (HAI < 10)	0	p > 0.99
WT viruses possessing inhibitor-sensitive HA3 and inhibitor- sensitive”NA3 (HAI = 2560-10240)	6:2 WT/MDV reassortants possessing inhibitor-sensitive HA3 and inhibitor-sensitive NA3 (HAI = 2560-10240)	0	p > 0.99
	7:1 reassortants possessing inhibitor-sensitive HA3 and inhibitor-resistant NA2 (HAI = 80-320)	11.09	p < 0.00001

1Sensitivity to non-specific, heat-stable inhibitors in guinea pig serum.

2Gene derived from a virus resistant to non-specific, heat-stable inhibitors in guinea pig serum.

3Gene derived from a virus sensitive to non-specific, heat-stable inhibitors in guinea pig serum.

4HAI: hemagglutination inhibition.

Table .5

Sensitivity of influenza viruses to HAI by normal guinea pig sera.

Virus Tested		Origin of Gene			HAI Titer	Sensitivity to Inhibitors
Code	Strain Designation (Subtype)	Type/Subtype	HA	NA
Influenza A Viruses
MDV1	A-MDV	H2N2	MDV	MDV	< 10	Resistant4
WT2	A/California/07/04	H3N2	WT	WT	2560	Sensitive5
R13	A/California/07/04 × MDV	H3N2	WT	MDV	320	Sensitive (<)7
R2	A/California/07/04 × MDV	H3N2	WT	WT	2560	Sensitive (=)7
WT	NIBRG-23	H5N1	WT	WT	5120	Sensitive
R3	NIBRG-23 × MDV	H5N2	WT	MDV	640	Sensitive (<)
R4	NIBRG-23 × MDV	H5N2	WT	MDV	640	Sensitive (<)
R5	NIBRG-23 × MDV	H5N2	WT	MDV	640	Sensitive (<)
WT	INDO/05	H5N1	WT	WT	10	Resistant
R6	INDO/05 × MDV	H5N2	WT	MDV	10	Resistant (=)
R7	INDO/05 × MDV	H5N2	WT	MDV	10	Resistant (=)
WT	VN1203	H5N1	WT	WT	< 10	Resistant
R8	VN1203 × MDV	H5N2	WT	MDV	< 10	Resistant (=)
R9	VN1203 × MDV	H5N2	WT	MDV	< 10	Resistant (=)
R106	A/Vietnam/1203/2004 × MDV	H5N2	WT	WT	< 10	Resistant (=)
Influenza B Viruses
MDV	B-MDV	B	MDV	MDV	< 10	Resistant
WT	B/Harbin/07/94	B	WT	WT	10240	Sensitive
R11	B/Harbin/07/94 × MDV	B	WT	MDV	2560	Sensitive (<)
WT	B/Texas/26/08	B	WT	WT	< 10	Resistant
R12	B/Texas/26/08 × MDV	B	WT	MDV	< 10	Resistant (=)
R13	B/Texas/26/08 × MDV	B	WT	WT	< 10	Resistant (=)
WT	B/Wisconsin/1/10	B	WT	WT	5120	Sensitive
R14	B/Wisconsin/1/10 × MDV	B	WT	MDV	320	Sensitive (<)
R15	B/Wisconsin/1/10 × MDV	B	WT	WT	5120	Sensitive (=)
R16	B/Wisconsin/1/10 × MDV	B	WT	WT	5120	Sensitive (=)
WT	B/Bangladesh/1994/10	B	WT	WT	5120	Sensitive
R17	B/Bangladesh/1994/10 × MDV	B	WT	MDV	640	Sensitive (<)
WT	B/Texas/06/11	B	WT	WT	640	Sensitive
R18	B/Texas/06/11 × MDV	B	WT	MDV	80	Sensitive (<)
R19	B/Texas/06/11 × MDV	B	WT	WT	640	Sensitive (=)

1MDV for LAIV: A/Leningrad/134/17/57 (H2N2) (A-MDV) or B/USSR/60/69 (B-MDV). 2WT virus or PR8-based virus inheriting HA and NA from WT virus. 3Reassortants of WT virus with MDV; the reassortants inherited HA from WT virus, and NA from WT virus (genomic composition 6:2) or MDV (genomic composition 7:1). 4Virus resistant to non-specific serum heat-stable inhibitors. 5Virus sensitive to non-specific serum heat-stable inhibitors. 66:2 R10 reassortant virus generated by plasmid-based reverse genetics as described in [14]. 7Sensitivity of reassortant virus to non-specific serum heat-stable inhibitors compared to WT parental virus; p < 0.0001).

Table 6.

Sequence differences in HA and NA of single gene influenza virus reassortants.

Parental Viruses		Reassortants			Substitutions in HA and NA Compared to Parental Virus
		Code	Origin of Gene Encoding		Nt Change		Amino Acid Change
WT or PR8-based virus	MDV		HA	NA	HA	NA	HA1	NA
A/California/07/04 (H3N2)	A-MDV1	R1	WT	MDV	no change	no change	no change	no change
NIBRG-23 (H5N1)	A-MDV	R3	WT	MDV	no change	G-765-T	no change	Arg-249-Ile
NIBRG-23 (H5N1)	A-MDV	R4	WT	MDV	no change	no change	no change	no change
NIBRG-23 (H5N1)	A-MDV	R5	WT	MDV	no change	no change	no change	no change
INDO/05 (H5N1)	A-MDV	R6	WT	MDV	no change	no change	no change	no change
INDO/05 (H5N1)	A-MDV	R7	WT	MDV	no change	no change	no change	no change
VN1203 (H5N1)	A-MDV	R8	WT	MDV	no change	no change	no change	no change
VN1203 (H5N1)	A-MDV	R9	WT	MDV	T-50-C T-417-C	G-765-T no change	no change Ile-114-Thr	Arg-249-Ile no change
B/Harbin/07/94	B-MDV2	R11	WT	MDV	no change	G-1085-A	no change	no change
B/Texas/26/08	B-MDV	R12	WT	MDV	no change	no change	no change	no change
B/Wisconsin/1/10	B-MDV	R14	WT	MDV	no change	G-1359-A	no change	Glu-436-Lys
B/Bangladesh/1994/10	B-MDV	R17	WT	MDV	no change	G-1085-A	no change	no change
B/Texas/06/11	B-MDV	R18	WT	MDV	no change	G-1349-A	no change	Glu-436-Lys

1MDV for LAIV: A/Leningrad/134/17/57 (H2N2). 2MDV for LAIV: B/USSR/60/69.

Table 7.

Origin of NA and PB2 genes of A/Leningrad/134/17/57 MDV reassortants with PR8-based strains for inactivated influenza vaccine.

PR8-based Strain for Inactivated Influenza Vaccine2	Reassortants1 of A/Leningrad/134/17/57 (H2N2) MDV with PR8-based Strains
	Total	Number (%) of Reassortants Inheriting PB2 and NA Gene Segments from MDV or WT Parental Virus2
		PB2 Gene From		NA Gene From
		PR8	MDV3	WT virus	MDV
NIBRG-23 (H5N1)4	17	0	17 (100%)	0	17 (100%)
	20	20 (100%)	0	20 (100%)	0
INDO/05 (H5N1)	11	0	11 (100%)	0	11 (100%)
VN1203 (H5N1)	28	0	28 (100%)	0	28 (100%)
	14	14 (100%)	0	14 (100%)	0
IRV-148 (H1N1)	285	0	28 (100%)	28 (100%)	0
NIB-64 (H3N2)	96	4 (44.4%)	5 (55.6%)	9 (100%)	0

1All reassortants contained HA from WT virus. The origin of PB1, PA, NP, M, and NS genes varied. 2PR8-based strains for inactivated influenza vaccine contain HA and NA from the appropriate WT virus, and the 6 internal genes from PR8 virus. 3MDV: A/Leningrad/134/17/57 (H2N2). 4Reassortants were generated after co-infecting MDV and NIBRG-23 (H5N1) virus inactivated by ultraviolet light. 56/28 IRV-148-based reassortants and 62/9 NIB-64-based reassortants were 6:2.