BACKGROUND: Diabetes mellitus is a chronicle illness in which there is a high blood glucose level defined as hyperglycemia, resulted by a deficiency in insulin secretion and/or in its action. Nowadays, it is being seen as a public health problem and is reaching increasing proportions with regard to the appearance of new cases. For diagnosis, sensible and accurate methods should be used to avoid complications of the sickness. The measure of glycated hemoglobin may not be used for diagnosis, but is the reference method to evaluate the grade of glycemic control in the long term, reflecting the blood glucose level in the latest 2-3 months. The aim of this study was to evaluate the grade of concordance between turbidimetry and liquid chromatography methods in the glycated hemoglobin determination and to estimate the sensibility and specificity values of turbidimetry. METHODS: This study included 133 blood samples obtained from patients and healthy donors, ageing between 18 and 80 years with glycemic values between 58 and 473 mg/dl. RESULTS AND CONCLUSION: Turbidimetry is a useful method for determining glycemic levels above 100 and over 200 mg/dl, but does not have the ability to select samples with intermediary blood glucose concentrations.
BACKGROUND:Diabetes mellitus is a chronicle illness in which there is a high blood glucose level defined as hyperglycemia, resulted by a deficiency in insulin secretion and/or in its action. Nowadays, it is being seen as a public health problem and is reaching increasing proportions with regard to the appearance of new cases. For diagnosis, sensible and accurate methods should be used to avoid complications of the sickness. The measure of glycated hemoglobin may not be used for diagnosis, but is the reference method to evaluate the grade of glycemic control in the long term, reflecting the blood glucose level in the latest 2-3 months. The aim of this study was to evaluate the grade of concordance between turbidimetry and liquid chromatography methods in the glycated hemoglobin determination and to estimate the sensibility and specificity values of turbidimetry. METHODS: This study included 133 blood samples obtained from patients and healthy donors, ageing between 18 and 80 years with glycemic values between 58 and 473 mg/dl. RESULTS AND CONCLUSION: Turbidimetry is a useful method for determining glycemic levels above 100 and over 200 mg/dl, but does not have the ability to select samples with intermediary blood glucose concentrations.
Authors: Nyoli A Valentine; Tariq M Alhawassi; Greg W Roberts; Parind P Vora; Parid P Vora; Stephen N Stranks; Matthew P Doogue Journal: Med J Aust Date: 2011-02-21 Impact factor: 7.738
Authors: Jeanethe Anguizola; Ryan Matsuda; Omar S Barnaby; K S Hoy; Chunling Wa; Erin DeBolt; Michelle Koke; David S Hage Journal: Clin Chim Acta Date: 2013-07-24 Impact factor: 3.786
Authors: Diewertje Sluik; Heiner Boeing; Jukka Montonen; Rudolf Kaaks; Annekatrin Lukanova; Annelli Sandbaek; Kim Overvad; Larraitz Arriola; Eva Ardanaz; Calogero Saieva; Sara Grioni; Rosario Tumino; Carlotta Sacerdote; Amalia Mattiello; Annemieke M W Spijkerman; Daphne L van der A; Joline W J Beulens; Susan van Dieren; Peter M Nilsson; Leif C Groop; Paul W Franks; Olov Rolandsson; Bas Bueno-de-Mesquita; Ute Nöthlings Journal: PLoS One Date: 2012-06-13 Impact factor: 3.240