Bradley C Campbell1, Edward K Gilding2, Victoria Timbrell3, Preethi Guru3, Dorothy Loo4, Danila Zennaro5, Adriano Mari6, Graham Solley7, Michelle M Hill4, Ian D Godwin1, Janet M Davies8. 1. School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Australia. 2. School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Australia; Institute for Molecular Biosciences, The University of Queensland, Brisbane, Australia. 3. School of Medicine, Translational Research Institute, The University of Queensland, Brisbane, Australia. 4. The University of Queensland Diamantina Institute, Translational Research Institute, The University of Queensland, Brisbane, Australia. 5. Centri Associati di Allergologia Molecolare, Rome, Italy. 6. Centri Associati di Allergologia Molecolare, Rome, Italy; Allergy Data Laboratories s.c., Latina, Italy. 7. Watkins Medical Centre, Brisbane, Australia. 8. School of Medicine, Translational Research Institute, The University of Queensland, Brisbane, Australia. Electronic address: j.davies2@uq.edu.au.
Abstract
BACKGROUND: Genomic data are lacking for many allergen sources. To circumvent this limitation, we implemented a strategy to reveal the repertoire of pollen allergens of a grass with clinical importance in subtropical regions, where an increasing proportion of the world's population resides. OBJECTIVE: We sought to identify and immunologically characterize the allergenic components of the Panicoideae Johnson grass pollen (JGP; Sorghum halepense). METHODS: The total pollen transcriptome, proteome, and allergome of JGP were documented. Serum IgE reactivities with pollen and purified allergens were assessed in 64 patients with grass pollen allergy from a subtropical region. RESULTS: Purified Sor h 1 and Sor h 13 were identified as clinically important allergen components of JGP with serum IgE reactivity in 49 (76%) and 28 (43.8%), respectively, of patients with grass pollen allergy. Within whole JGP, multiple cDNA transcripts and peptide spectra belonging to grass pollen allergen families 1, 2, 4, 7, 11, 12, 13, and 25 were identified. Pollen allergens restricted to subtropical grasses (groups 22-24) were also present within the JGP transcriptome and proteome. Mass spectrometry confirmed the IgE-reactive components of JGP included isoforms of Sor h 1, Sor h 2, Sor h 13, and Sor h 23. CONCLUSION: Our integrated molecular approach revealed qualitative differences between the allergenic components of JGP and temperate grass pollens. Knowledge of these newly identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with grass pollen allergy in subtropical regions and reduce the burden of allergic respiratory disease globally.
BACKGROUND: Genomic data are lacking for many allergen sources. To circumvent this limitation, we implemented a strategy to reveal the repertoire of pollen allergens of a grass with clinical importance in subtropical regions, where an increasing proportion of the world's population resides. OBJECTIVE: We sought to identify and immunologically characterize the allergenic components of the Panicoideae Johnson grass pollen (JGP; Sorghum halepense). METHODS: The total pollen transcriptome, proteome, and allergome of JGP were documented. Serum IgE reactivities with pollen and purified allergens were assessed in 64 patients with grass pollen allergy from a subtropical region. RESULTS: Purified Sor h 1 and Sor h 13 were identified as clinically important allergen components of JGP with serum IgE reactivity in 49 (76%) and 28 (43.8%), respectively, of patients with grass pollen allergy. Within whole JGP, multiple cDNA transcripts and peptide spectra belonging to grass pollen allergen families 1, 2, 4, 7, 11, 12, 13, and 25 were identified. Pollen allergens restricted to subtropical grasses (groups 22-24) were also present within the JGP transcriptome and proteome. Mass spectrometry confirmed the IgE-reactive components of JGP included isoforms of Sor h 1, Sor h 2, Sor h 13, and Sor h 23. CONCLUSION: Our integrated molecular approach revealed qualitative differences between the allergenic components of JGP and temperate grass pollens. Knowledge of these newly identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with grass pollen allergy in subtropical regions and reduce the burden of allergic respiratory disease globally.
Authors: Anna Pomés; Janet M Davies; Gabriele Gadermaier; Christiane Hilger; Thomas Holzhauser; Jonas Lidholm; Andreas L Lopata; Geoffrey A Mueller; Andreas Nandy; Christian Radauer; Sanny K Chan; Uta Jappe; Jörg Kleine-Tebbe; Wayne R Thomas; Martin D Chapman; Marianne van Hage; Ronald van Ree; Stefan Vieths; Monika Raulf; Richard E Goodman Journal: Mol Immunol Date: 2018-04-04 Impact factor: 4.407