Qing-Ke Cui1, Wei-Dong Liu2, Jian-Xin Zhu2, Yun-Hua Wang2, Zhi-Gang Wang3. 1. Department of Neurosurgery, The Qilu Hospital of Shandong University, Jinan, Shandong, 250012, P. R. China; Liaocheng People's Hospital, Liaocheng, Shandong, 252000, P. R. China. 2. Liaocheng People's Hospital, Liaocheng, Shandong, 252000, P. R. China. 3. Department of Neurosurgery, The Qilu Hospital of Shandong University, Jinan, Shandong, 250012, P. R. China. Electronic address: wangzgt1962@163.com.
Abstract
OBJECTIVES: To investigate the effect of microRNA (miR-184) on regulating the genesis, development and proliferation of glioma cells. METHODS: Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell line, and the cell proliferation ability changes were determined by MTT and plate cloning experiment after the transfection. WB test was used to measure the levels of cyclinD1, p27 and FOXO3. Meanwhile, QPCR was used to detect miR-184 expression in glioma cell line, glioma tissues and adjacent tissues. Luciferase experiment was used to test 3'UTR gene targeting regulation of miR-184 and FOXO3. RESULTS: QPCR results showed a significant lower miR-184 expression level in glioma cell line and glioma tissues than that in juxtacancerous tissue. MTT and plate cloning experiments have shown that after over-expressing of miR-184, the cell proliferation capacity of glioma U87 and T98G was significantly increased, which was significantly inhibited after the inhibition of miR-184. WB results showed a lower expression level of p27 in U87 and T98G cells, and a higher expression level of cyclinD1 after over-expressing of miR-184 was observed. However, a lower expression level of cyclinD1 and a higher expression level of p27 after the inhibition of miR-184. The luciferase activity was inhibited after the over-expressing of miR-184. CONCLUSIONS: MiR-184 can affect the proliferation abilities of glioma cells and regulate the cell cycle related protein. It plays an important role in the occurrence and development of gliomas.
OBJECTIVES: To investigate the effect of microRNA (miR-184) on regulating the genesis, development and proliferation of glioma cells. METHODS: Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell line, and the cell proliferation ability changes were determined by MTT and plate cloning experiment after the transfection. WB test was used to measure the levels of cyclinD1, p27 and FOXO3. Meanwhile, QPCR was used to detect miR-184 expression in glioma cell line, glioma tissues and adjacent tissues. Luciferase experiment was used to test 3'UTR gene targeting regulation of miR-184 and FOXO3. RESULTS: QPCR results showed a significant lower miR-184 expression level in glioma cell line and glioma tissues than that in juxtacancerous tissue. MTT and plate cloning experiments have shown that after over-expressing of miR-184, the cell proliferation capacity of glioma U87 and T98G was significantly increased, which was significantly inhibited after the inhibition of miR-184. WB results showed a lower expression level of p27 in U87 and T98G cells, and a higher expression level of cyclinD1 after over-expressing of miR-184 was observed. However, a lower expression level of cyclinD1 and a higher expression level of p27 after the inhibition of miR-184. The luciferase activity was inhibited after the over-expressing of miR-184. CONCLUSIONS:MiR-184 can affect the proliferation abilities of glioma cells and regulate the cell cycle related protein. It plays an important role in the occurrence and development of gliomas.
Authors: Laila Al-Eryani; Samantha F Jenkins; Vanessa A States; Jianmin Pan; Janine C Malone; Shesh N Rai; Susan Galandiuk; Ashok K Giri; J Christopher States Journal: PLoS One Date: 2018-08-16 Impact factor: 3.240