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Literature DB >> 25126958

Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin.

Hiroshi Itou¹, Sachiko Muramatsu², Yasuo Shirakihara³, Hiroyuki Araki⁴.

Abstract

The initiation of eukaryotic chromosomal DNA replication requires the formation of an active replicative helicase at the replication origins of chromosomal DNA. Yeast Sld3 and its metazoan counterpart Treslin are the hub proteins mediating protein associations critical for the helicase formation. Here, we show the crystal structure of the central domain of Sld3 that is conserved in Sld3/Treslin family of proteins. The domain consists of two segments with 12 helices and is sufficient to bind to Cdc45, the essential helicase component. The structure model of the Sld3-Cdc45 complex, which is crucial for the formation of the active helicase, is proposed.

Entities: Gene Species

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Year: 2014 PMID： 25126958 DOI： 10.1016/j.str.2014.07.001

Source DB: PubMed Journal: Structure ISSN： 0969-2126 Impact factor: 5.006

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Cited

13 in total

1. Human DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.

Authors: Jeannine Gerhardt; Gulfem D Guler; Ellen Fanning
Journal: Exp Cell Res Date: 2015-04-29 Impact factor: 3.905

2. Elucidating the DDK-dependent step in replication initiation.

Authors: Hiroyuki Araki
Journal: EMBO J Date: 2016-03-22 Impact factor: 11.598

3. Interaction of replication factor Sld3 and histone acetyl transferase Esa1 alleviates gene silencing and promotes the activation of late and dormant replication origins.

Authors: Seiji Tanaka
Journal: Genetics Date: 2021-03-03 Impact factor: 4.562

Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin.

1. Human DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.

2. Elucidating the DDK-dependent step in replication initiation.

3. Interaction of replication factor Sld3 and histone acetyl transferase Esa1 alleviates gene silencing and promotes the activation of late and dormant replication origins.

4. Insights into the Initiation of Eukaryotic DNA Replication.

5. The Dbf4-Cdc7 kinase promotes Mcm2-7 ring opening to allow for single-stranded DNA extrusion and helicase assembly.

Review 6. Evolutionary conservation of the CDK targets in eukaryotic DNA replication initiation.

7. Regulated eukaryotic DNA replication origin firing with purified proteins.

8. Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation.

9. Structure of human Cdc45 and implications for CMG helicase function.

Review 10. Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.