Maohua Chen1, Zhenghao Liu2, Yuefei Deng2, Xianglin Chen3, Jianmin Zhang4. 1. School of Medicine, Zhejiang University & Department of Neurosurgery, Wenzhou Central Hospital Zhejiang, China. 2. Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou, China. 3. Department of Neurosurgery, Qingyuan People's Hospital Guangdong, China. 4. Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Zhejiang, China.
Abstract
OBJECTIVE: This study aimed to investigate the methylation status of promoter 1 region of glial cell line-derived neurotrophic factor (GDNF) in human glioma cells and to explore the effect of GDNF methylation on the expression of GDNF in glioma. METHODS: GDNF gene mutation was detected by sequencing in 10 patients with glioma and 5 healthy controls. Bisulfite modification for analysis of DNA methylation was done to detect the methylation status of promoter 1 region of GDNF in 20 patients with glioma (10 with poorly differentiated and 10 with well differentiated) and 5 healthy controls. RESULTS: There was no mutation at the promoter 1 region of GDNF gene in glioma. The incidence of methylation of GDNF gene at the promoter 1 region in healthy control, patients with poorly differentiated glioma and those with well differentiated glioma was 72.25%, 86.25% and 86.75%. The incidence of GDNF methylation in glioma was significantly higher than that in the normal brain (P<0.05); while there was no significant difference between well differentiated glioma and poorly differentiated glioma. CONCLUSIONS: Hypermethylation occurs in the promoter 1 region of GDNF and may influence the expression of GDNF in glioma.
OBJECTIVE: This study aimed to investigate the methylation status of promoter 1 region of glial cell line-derived neurotrophic factor (GDNF) in humanglioma cells and to explore the effect of GDNF methylation on the expression of GDNF in glioma. METHODS:GDNF gene mutation was detected by sequencing in 10 patients with glioma and 5 healthy controls. Bisulfite modification for analysis of DNA methylation was done to detect the methylation status of promoter 1 region of GDNF in 20 patients with glioma (10 with poorly differentiated and 10 with well differentiated) and 5 healthy controls. RESULTS: There was no mutation at the promoter 1 region of GDNF gene in glioma. The incidence of methylation of GDNF gene at the promoter 1 region in healthy control, patients with poorly differentiated glioma and those with well differentiated glioma was 72.25%, 86.25% and 86.75%. The incidence of GDNF methylation in glioma was significantly higher than that in the normal brain (P<0.05); while there was no significant difference between well differentiated glioma and poorly differentiated glioma. CONCLUSIONS: Hypermethylation occurs in the promoter 1 region of GDNF and may influence the expression of GDNF in glioma.
Authors: Jennie E Minnich; Sarah L Mann; Megan Stock; Kathryn A Stolzenbach; Bridget M Mortell; Katherine E Soderstrom; Martha C Bohn; Dorothy A Kozlowski Journal: Restor Neurol Neurosci Date: 2010 Impact factor: 2.406
Authors: Christophe Morisseau; John W Newman; Hsing-Ju Tsai; Preston A Baecker; Bruce D Hammock Journal: Bioorg Med Chem Lett Date: 2006-10-15 Impact factor: 2.823