Bora Aktaş1, Omer Başar2, Barış Yılmaz1, Fuat Ekiz1, Akif Altınbaş1, Sahin Coban1, Fatih Büyükçam3, Aynur Albayrak4, Zeynep Giniş5, Osman Yüksel2, Tuncay Delibaşı6. 1. Department of Gastroenterology, Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey. 2. Department of Gastroenterology, Hacettepe Universty Medical School Ankara, Turkey. 3. Department of Emergency Medicine, Ankara Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey. 4. Department of Pathology, Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey. 5. Department of Biochemistry, Ankara Dışkapı Yıldırım Beyazıt Training and Research Hospital Ankara, Turkey. 6. Department of Endocrinology, Hacettepe Universty Medical School Ankara, Turkey.
Abstract
BACKGROUND AND AIM: The incidence of acute pancreatitis is increasing recently. The aim of this study is to investigate the effect of Ankaferd Blood Stopper (ABS) on experimental model of cerulein induced acute pancreatitis in rats. MATERIALS AND METHODS: Forty Wistar Albino rats were divided into five groups. Group 1: Sham (n = 8), Group 2: Control group (n = 8), Group 3: Treatment group (n = 8), Group 4: Prophylaxis group (n = 8), Group 5: Prophylaxis treatment group (n = 8). Any practice was not administered to Group 1. Rats were treated with either 1 ml ABS or 1 ml saline via intraperitoneal route before and after inducing acute pancreatitis. Pancreatic tissues were examined histopathologically. Amylase, cytokines (tumor necrosis factor-α and interleukin-1β), and markers of apoptosis (M30 and M65) were also measured in blood samples. Immunohistochemical staining was performed with caspase 3 antibody. RESULTS: We found a statistically significant improvement in histopathological scores in treatment group and prophylaxis group compared with controls. In treatment group, M30 and M65 levels were lower when compared with controls. In prophylaxis group, there was not a statistically significant difference in M30 levels, but M65 levels were lower when compared with controls. CONCLUSION: In this experimental acute pancreatitis model, we found high histopathological healing effects of ABS treatment and also prophylaxis. ABS treatment and prophylaxis reduced apoptosis.
BACKGROUND AND AIM: The incidence of acute pancreatitis is increasing recently. The aim of this study is to investigate the effect of Ankaferd Blood Stopper (ABS) on experimental model of cerulein induced acute pancreatitis in rats. MATERIALS AND METHODS: Forty Wistar Albino rats were divided into five groups. Group 1: Sham (n = 8), Group 2: Control group (n = 8), Group 3: Treatment group (n = 8), Group 4: Prophylaxis group (n = 8), Group 5: Prophylaxis treatment group (n = 8). Any practice was not administered to Group 1. Rats were treated with either 1 ml ABS or 1 ml saline via intraperitoneal route before and after inducing acute pancreatitis. Pancreatic tissues were examined histopathologically. Amylase, cytokines (tumor necrosis factor-α and interleukin-1β), and markers of apoptosis (M30 and M65) were also measured in blood samples. Immunohistochemical staining was performed with caspase 3 antibody. RESULTS: We found a statistically significant improvement in histopathological scores in treatment group and prophylaxis group compared with controls. In treatment group, M30 and M65 levels were lower when compared with controls. In prophylaxis group, there was not a statistically significant difference in M30 levels, but M65 levels were lower when compared with controls. CONCLUSION: In this experimental acute pancreatitis model, we found high histopathological healing effects of ABS treatment and also prophylaxis. ABS treatment and prophylaxis reduced apoptosis.
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