Da-Hyun Jeong1, Koth-Bong-Woo-Ri Kim2, Min-Ji Kim2, Bo-Kyeong Kang1, Dong-Hyun Ahn3. 1. Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea. 2. Institute of Fisheries Sciences, Pukyong National University, Busan 619-911, Republic of Korea. 3. Department of Food Science & Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: dhahn@pknu.ac.kr.
Abstract
AIMS: This study was carried out to verify the anti-inflammatory effect of methanol extract from Myagropsis myagroides (MMME) and its n-hexane fraction mojabanchromanol b. MAIN METHODS: The murine macrophages Raw264.7 cells were used. The pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) and the expression of iNOS, COX-2, and NF-κB p65 were examined by ELISA and immunoblotting. To investigate the inhibitory effect of MMME in an animal model of inflammation, an assay to determine croton oil-induced ear edema in mice was performed. KEY FINDINGS: NO levels decreased with increasing concentration of MMME, and were inhibited up to 50%. The secretion of IL-6, TNF-α, and IL-1β was suppressed in a dose-dependent manner, especially at 50μg/mL, inhibition activities of cytokines were over 50%. MMME also suppressed the expression of COX-2, iNOS, and NF-κB p65, suggesting that MMME could affect the expression of inflammation related cytokines and proteins through the deregulation of NF-κB. Moreover, the formation of mouse ear edema was reduced at the highest dose tested compared to that in the control, and generated similar effects compared with prednisolone at 250mg/kg in mice ear edema evaluation test. In addition, the results in photomicrograph of mice ear tissue and mast cells also showed the same effect. After purification of fractions of MMME, it indicated that n-hexane fraction mojabanchromanol b was the most active fraction showing the inhibitory effect of IL-6 and TNF-α. SIGNIFICANCE: These results suggested that MMME and mojabanchromanol b may have great effects on inflammatory factors and be potential anti-inflammatory therapeutic materials.
AIMS: This study was carried out to verify the anti-inflammatory effect of methanol extract from Myagropsis myagroides (MMME) and its n-hexane fraction mojabanchromanol b. MAIN METHODS: The murine macrophages Raw264.7 cells were used. The pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) and the expression of iNOS, COX-2, and NF-κB p65 were examined by ELISA and immunoblotting. To investigate the inhibitory effect of MMME in an animal model of inflammation, an assay to determine croton oil-induced ear edema in mice was performed. KEY FINDINGS: NO levels decreased with increasing concentration of MMME, and were inhibited up to 50%. The secretion of IL-6, TNF-α, and IL-1β was suppressed in a dose-dependent manner, especially at 50μg/mL, inhibition activities of cytokines were over 50%. MMME also suppressed the expression of COX-2, iNOS, and NF-κB p65, suggesting that MMME could affect the expression of inflammation related cytokines and proteins through the deregulation of NF-κB. Moreover, the formation of mouseear edema was reduced at the highest dose tested compared to that in the control, and generated similar effects compared with prednisolone at 250mg/kg in miceear edema evaluation test. In addition, the results in photomicrograph of mice ear tissue and mast cells also showed the same effect. After purification of fractions of MMME, it indicated that n-hexane fraction mojabanchromanol b was the most active fraction showing the inhibitory effect of IL-6 and TNF-α. SIGNIFICANCE: These results suggested that MMME and mojabanchromanol b may have great effects on inflammatory factors and be potential anti-inflammatory therapeutic materials.
Authors: Cleydlenne Costa Vasconcelos; Alberto Jorge Oliveira Lopes; Emilly de Jesus Garcia Ataide; Kevin Waquim Pessoa Carvalho; Maria Fernanda Freitas de Brito; Marineide Sodré Rodrigues; Sebastião Vieira de Morais; Gyl Eanes Barros Silva; Claudia Quintino da Rocha; João Batista Santos Garcia; Maria do Socorro de Sousa Cartágenes Journal: Inflammopharmacology Date: 2021-04-21 Impact factor: 4.473