Literature DB >> 2511557

A novel method for the purification of the Xenopus transcription factor IIIA.

J Miller1, L Fairall, D Rhodes.   

Abstract

We have developed a quick and simple purification method that yields large quantities of the Xenopus zinc finger protein transcription factor IIIA (TFIIIA). The protein is purified in the form of the 7S storage particle (TFIIIA/5S RNA complex) found in the ovaries of immature Xenopus. Our method yields 0.5 to 1 mg of pure 7S particle per ovary. It involves a high speed centrifugation step, fractionation on a gel filtration column and a precipitation step using calcium chloride. TFIIIA purified using this protocol retains full DNA binding activity and has the expected zinc ion content.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2511557      PMCID： PMC335123          DOI： 10.1093/nar/17.22.9185

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Crystallization of nucleosome core particles.

Authors:  D Rhodes; R S Brown; A Klug
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

2.  Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.

Authors:  D R Engelke; S Y Ng; B S Shastry; R G Roeder
Journal:  Cell       Date:  1980-03       Impact factor: 41.582

3.  Isolation of a 7S particle from Xenopus laevis oocytes: a 5S RNA-protein complex.

Authors:  B Picard; M Wegnez
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

4.  Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.

Authors:  A M Ginsberg; B O King; R G Roeder
Journal:  Cell       Date:  1984-12       Impact factor: 41.582

5.  Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.

Authors:  D R Smith; I J Jackson; D D Brown
Journal:  Cell       Date:  1984-06       Impact factor: 41.582

6.  A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.

Authors:  H R Pelham; D D Brown
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

7.  Xenopus transcription factor A requires zinc for binding to the 5 S RNA gene.

Authors:  J S Hanas; D J Hazuda; D F Bogenhagen; F Y Wu; C W Wu
Journal:  J Biol Chem       Date:  1983-12-10       Impact factor: 5.157

8.  Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.

Authors:  J Miller; A D McLachlan; A Klug
Journal:  EMBO J       Date:  1985-06       Impact factor: 11.598

9.  Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.

Authors:  D Rhodes
Journal:  EMBO J       Date:  1985-12-16       Impact factor: 11.598
View more
  3 in total

Review 1.  Zinc in specialized secretory tissues: roles in the pancreas, prostate, and mammary gland.

Authors:  Shannon L Kelleher; Nicholas H McCormick; Vanessa Velasquez; Veronica Lopez
Journal:  Adv Nutr       Date:  2011-03-10       Impact factor: 8.701

2.  Binding of TFIIIA to derivatives of 5S RNA containing sequence substitutions or deletions defines a minimal TFIIIA binding site.

Authors:  D F Bogenhagen; M S Sands
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

3.  Mode of interaction of the zinc finger protein TFIIIA with a 5S RNA gene of Xenopus.

Authors:  M E Churchill; T D Tullius; A Klug
Journal:  Proc Natl Acad Sci U S A       Date:  1990-07       Impact factor: 11.205
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.