Literature DB >> 25111966

Characterization of recombinant malarial RecQ DNA helicase.

Pattra Suntornthiticharoen1, Witsanu Srila2, Porntip Chavalitshewinkoon-Petmitr3, Paviga Limudomporn3, Montarop Yamabhai4.   

Abstract

RecQ DNA gene of multi-drug resistant Plasmodium falciparum K1 (PfRecQ1) was cloned, and the recombinant C-terminal-decahistidine-tagged PfRecQ1 was expressed in Escherichia coli. The purified enzyme could efficiently unwind partial duplex DNA substrate in a 3' to 5' direction. The malarial RecQ1 could not unwind substrates with both 5' and 3' overhangs, those with a 5' overhang, or blunt-ended DNA duplexes. Unwinding of DNA helicase activity was driven by the hydrolysis of ATP. The drug inhibitory effects of six compounds indicated that only doxorubicin and daunorubicin could inhibit the unwinding activity.
Copyright © 2014 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Cloning; DNA helicase; Expression; Malaria; Plasmodium falciparum; RecQ

Mesh：

Substances：

Year:  2014        PMID： 25111966     DOI： 10.1016/j.molbiopara.2014.07.013

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  5 in total

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Authors:  Farhana Rahman; Mohammed Tarique; Moaz Ahmad; Renu Tuteja
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2.  Inhibitory effects of anthracyclines on partially purified 5'-3' DNA helicase of Plasmodium falciparum.

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3.  Recombination events among virulence genes in malaria parasites are associated with G-quadruplex-forming DNA motifs.

Authors:  Adam Stanton; Lynne M Harris; Gemma Graham; Catherine J Merrick
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4.  Elucidation of DNA Repair Function of PfBlm and Potentiation of Artemisinin Action by a Small-Molecule Inhibitor of RecQ Helicase.

Authors:  Niranjan Suthram; Siladitya Padhi; Payal Jha; Sunanda Bhattacharyya; Gopalakrishnan Bulusu; Arijit Roy; Mrinal Kanti Bhattacharyya
Journal:  mSphere       Date:  2020-11-25       Impact factor: 4.389

5.  RecQ helicases in the malaria parasite Plasmodium falciparum affect genome stability, gene expression patterns and DNA replication dynamics.

Authors:  Antoine Claessens; Lynne M Harris; Slavica Stanojcic; Lia Chappell; Adam Stanton; Nada Kuk; Pamela Veneziano-Broccia; Yvon Sterkers; Julian C Rayner; Catherine J Merrick
Journal:  PLoS Genet       Date:  2018-07-02       Impact factor: 5.917
  5 in total

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