Matthew K Lee1, Adam S DeConde1, Min Lee2, Christopher M Walthers3, Ali R Sepahdari4, David Elashoff5, Tristan Grogan5, Olga Bezouglaia6, Sotirios Tetradis7, Maie St John8, Tara Aghaloo9. 1. Department of Head and Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States. 2. Division of Advanced Prosthodontics, Biomaterials, and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, CA, United States; Department of Bioengineering, UCLA School of Engineering and Applied Sciences, Los Angeles, CA, United States. 3. Department of Bioengineering, UCLA School of Engineering and Applied Sciences, Los Angeles, CA, United States. 4. Department of Radiology, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States. 5. Department of Medicine Statistics Core, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States. 6. Division of Diagnostic and Surgical Sciences, UCLA School of Dentistry, Los Angeles, CA, United States. 7. Division of Diagnostic and Surgical Sciences, UCLA School of Dentistry, Los Angeles, CA, United States; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States. 8. Department of Head and Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States. Electronic address: mstjohn@mednet.ucla.edu. 9. Division of Diagnostic and Surgical Sciences, UCLA School of Dentistry, Los Angeles, CA, United States; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States. Electronic address: taghaloo@ucla.edu.
Abstract
OBJECTIVE: To investigate the efficacy of biomimetic PLGA scaffolds, alone and in combination with bone morphogenic protein (BMP-2) and adipose-derived stem cells (ASCs), to heal a critical-sized segmental mandibular defect in a rat model. STUDY DESIGN: Prospective animal study. METHODS: ASCs were isolated and cultured from the inguinal fat of Lewis rat pups. Using three-dimensional printing, PLGA scaffolds were fabricated and impregnated with BMP-2 and/or ASCs. Critical-sized 5-mm segmental mandibular defects were created in adult Lewis rats and implanted with (1) blank PLGA scaffolds, (2) PLGA scaffolds with ASCs, (3) PLGA scaffolds with BMP, or (4) PLGA scaffolds with BMP and ASCs. Animals were sacrificed at 12weeks. Bone regeneration was assessed using microCT, and graded on a semi-quantitative bone formation and bone union scale. RESULTS: Twenty-eight rats underwent creation of segmental mandibular defects with implantation of scaffolds. Nine rats suffered complications and were excluded from analysis, leaving 19 animals for inclusion in the study. MicroCT analysis demonstrated no bridging of the segmental bony defect in rats implanted with blank scaffolds (median bone union score=0). Rats implanted with scaffolds containing BMP-2 (median bone union=2.0), ASCs (median bone union=1.5), and combination of BMP and ASCs (median bone union=1.0) demonstrated healing of critical-sized segmental mandibular defects. Bone regeneration was most robust in the BMP-2 treated scaffolds. CONCLUSIONS: The current study utilizes a novel animal model to study the efficacy of biomimetic scaffolds carrying osteogenic factors to induce healing of a critical-sized segmental mandibular defect. LEVEL OF EVIDENCE: N/A, Basic Science Animal Research.
OBJECTIVE: To investigate the efficacy of biomimetic PLGA scaffolds, alone and in combination with bone morphogenic protein (BMP-2) and adipose-derived stem cells (ASCs), to heal a critical-sized segmental mandibular defect in a rat model. STUDY DESIGN: Prospective animal study. METHODS: ASCs were isolated and cultured from the inguinal fat of Lewis rat pups. Using three-dimensional printing, PLGA scaffolds were fabricated and impregnated with BMP-2 and/or ASCs. Critical-sized 5-mm segmental mandibular defects were created in adult Lewis rats and implanted with (1) blank PLGA scaffolds, (2) PLGA scaffolds with ASCs, (3) PLGA scaffolds with BMP, or (4) PLGA scaffolds with BMP and ASCs. Animals were sacrificed at 12weeks. Bone regeneration was assessed using microCT, and graded on a semi-quantitative bone formation and bone union scale. RESULTS: Twenty-eight rats underwent creation of segmental mandibular defects with implantation of scaffolds. Nine rats suffered complications and were excluded from analysis, leaving 19 animals for inclusion in the study. MicroCT analysis demonstrated no bridging of the segmental bony defect in rats implanted with blank scaffolds (median bone union score=0). Rats implanted with scaffolds containing BMP-2 (median bone union=2.0), ASCs (median bone union=1.5), and combination of BMP and ASCs (median bone union=1.0) demonstrated healing of critical-sized segmental mandibular defects. Bone regeneration was most robust in the BMP-2 treated scaffolds. CONCLUSIONS: The current study utilizes a novel animal model to study the efficacy of biomimetic scaffolds carrying osteogenic factors to induce healing of a critical-sized segmental mandibular defect. LEVEL OF EVIDENCE: N/A, Basic Science Animal Research.
Authors: Ram Alluri; Adam Jakus; Sofia Bougioukli; William Pannell; Osamu Sugiyama; Amy Tang; Ramille Shah; Jay R Lieberman Journal: J Biomed Mater Res A Date: 2018-01-11 Impact factor: 4.396
Authors: Matthew Q Miller; Logan F McColl; Michael R Arul; Jonathan Nip; Vedavathi Madhu; Gina Beck; Kishan Mathur; Vashaana Sahadeo; Jason R Kerrigan; Stephen S Park; J Jared Christophel; Abhijit S Dighe; Sangamesh G Kumbar; Quanjun Cui Journal: JAMA Facial Plast Surg Date: 2019-03-01 Impact factor: 4.611
Authors: Andrew Wofford; Austin Bow; Steven Newby; Seth Brooks; Rachel Rodriguez; Tom Masi; Stacy Stephenson; Jack Gotcher; David E Anderson; Josh Campbell; Madhu Dhar Journal: Stem Cells Int Date: 2020-01-13 Impact factor: 5.443