Jian Wu1, Peng Li, Xiuying Wu, Weimin Chen. 1. Department of Anesthesiology, Shengjing Hospital, China Medical University, No. 36 Sanhao Street, Heping District, Shenyang, 110004, China.
Abstract
PURPOSE: The present study was designed to determine the effects of chronic intermittent hypoxia (CIH) on pain sensitivity and the changes in the expression of delta-opioid receptor (DOR), mu-opioid receptor (MOR), and hypoxia-inducible factor (HIF)-1α and their relationship with pain sensitivity under hypoxia. METHODS: CIH was employed to model the low oxygen condition in obstructive sleep apnea (OSA). Normal oxygen condition was used as control. After 1-, 2-, 3-, and 4-week hypoxia, behavioral tests, including paw-withdrawal latency (PWL) and paw-withdrawal threshold (PWT), were performed. The expressions of DOR, MOR and HIF-1α in cortex were analyzed by real-time PCR and Western blotting. RESULTS: HIF-1α expression was significantly upregulated after 1-4 weeks of hypoxia at both mRNA and protein levels, compared with the controls (P < 0.05). Both PWL and PWT were significantly enhanced in the rats following 3 and 4 weeks of hypoxia (P < 0.05). DOR and MOR expression was significantly upregulated at both mRNA and protein levels after 3 and 4 weeks of hypoxia (P < 0.05), which corresponded to the behavioral changes. CONCLUSIONS: CIH decreases pain sensitivity in rats, possibly through activating HIF-1α and increasing MOR and DOR expression.
PURPOSE: The present study was designed to determine the effects of chronic intermittent hypoxia (CIH) on pain sensitivity and the changes in the expression of delta-opioid receptor (DOR), mu-opioid receptor (MOR), and hypoxia-inducible factor (HIF)-1α and their relationship with pain sensitivity under hypoxia. METHODS:CIH was employed to model the low oxygen condition in obstructive sleep apnea (OSA). Normal oxygen condition was used as control. After 1-, 2-, 3-, and 4-week hypoxia, behavioral tests, including paw-withdrawal latency (PWL) and paw-withdrawal threshold (PWT), were performed. The expressions of DOR, MOR and HIF-1α in cortex were analyzed by real-time PCR and Western blotting. RESULTS: HIF-1α expression was significantly upregulated after 1-4 weeks of hypoxia at both mRNA and protein levels, compared with the controls (P < 0.05). Both PWL and PWT were significantly enhanced in the rats following 3 and 4 weeks of hypoxia (P < 0.05). DOR and MOR expression was significantly upregulated at both mRNA and protein levels after 3 and 4 weeks of hypoxia (P < 0.05), which corresponded to the behavioral changes. CONCLUSIONS:CIH decreases pain sensitivity in rats, possibly through activating HIF-1α and increasing MOR and DOR expression.
Authors: F Simonin; O Valverde; C Smadja; S Slowe; I Kitchen; A Dierich; M Le Meur; B P Roques; R Maldonado; B L Kieffer Journal: EMBO J Date: 1998-02-16 Impact factor: 11.598
Authors: Dan Kaufmann; Boris Yagen; Anne Minert; Michael Tal; Marshall Devor; Meir Bialer Journal: Neuropharmacology Date: 2007-12-08 Impact factor: 5.250
Authors: Michael T Smith; Brendan Klick; Sharon Kozachik; Robert E Edwards; Radha Holavanahalli; Shelley Wiechman; Patricia Blakeney; Dennis Lezotte; James A Fauerbach Journal: Pain Date: 2008-03-24 Impact factor: 6.961
Authors: Anthony G Doufas; Lu Tian; Kevin A Padrez; Puntarica Suwanprathes; James A Cardell; Holden T Maecker; Periklis Panousis Journal: PLoS One Date: 2013-01-29 Impact factor: 3.240
Authors: Agata Gabryelska; Bartosz Szmyd; Janusz Szemraj; Robert Stawski; Marcin Sochal; Piotr Białasiewicz Journal: J Clin Sleep Med Date: 2020-10-15 Impact factor: 4.062
Authors: Nicholas M Dalesio; Carlton K K Lee; Craig W Hendrix; Nikole Kerns; Aaron Hsu; William Clarke; Joseph M Collaco; Sharon McGrath-Morrow; Myron Yaster; Robert H Brown; Alan R Schwartz Journal: Anesth Analg Date: 2020-09 Impact factor: 6.627
Authors: Piotr Kaczmarski; Filip Franciszek Karuga; Bartosz Szmyd; Marcin Sochal; Piotr Białasiewicz; Dominik Strzelecki; Agata Gabryelska Journal: Int J Mol Sci Date: 2022-08-13 Impact factor: 6.208