| Literature DB >> 25108205 |
Wei Li1, Guang-Bin Jiang1, Jun-Hua Yao2, Xiu-Zhen Wang1, Ji Wang1, Bing-Jie Han1, Yang-Yin Xie1, Gan-Jian Lin1, Hong-Liang Huang3, Yun-Jun Liu4.
Abstract
The aim of our study was to investigate DNA-binding and cytotoxic activity of the four new Ru(II) polypyridyl complexes [Ru(dmb)₂(HMHPIP)](ClO₄)₂ (1), [Ru(bpy)₂(HMHPIP)](ClO₄)₂ (2), [Ru(phen)₂(HMHPIP)](ClO₄)₂ (3) and [Ru(dmp)₂(HMHPIP)](ClO₄)₂ (4). The complexes interact with DNA through intercalative mode and show relatively high cytotoxic activity against A549 cells, no cytotoxicity toward MG-63 cells. Complexes 1-4 can enhance the levels of ROS in A549 cells and induce the decrease of the mitochondrial membrane potential. These complexes inhibit the cell growth in A549 cells at G0/G1 or S phase. Complex 3 activated caspase 7, and down-regulated the expression of the anti-apoptotic protein Bcl-2. Complexes 1-4 induce apoptosis in A549 cells through ROS-mediated mitochondrial dysfunction pathway. CrownEntities:
Keywords: Cell cycle arrest; Cytotoxicity in vitro; DNA-binding; Reactive oxygen species; Ru(II) polypyridyl complex; Western blot analysis
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Year: 2014 PMID： 25108205 DOI： 10.1016/j.jphotobiol.2014.07.011
Source DB: PubMed Journal: J Photochem Photobiol B ISSN： 1011-1344 Impact factor: 6.252