Emily Vogtmann1, Gong Yang1, Hong-Lan Li2, Jing Wang2, Li-Hua Han2, Qi-Jun Wu2, Li Xie2, Quiyin Cai1, Guo-Liang Li1, John W Waterbor3, Emily B Levitan3, Bin Zhang4, Yu-Tang Gao2, Wei Zheng1, Yong-Bing Xiang2, Xiao-Ou Shu1. 1. 1Division of Epidemiology,Department of Medicine,Vanderbilt Epidemiology Center,Vanderbilt-Ingram Cancer Center,Vanderbilt University School of Medicine,2525 West End Avenue 6th floor,Nashville,TN 37232-8300,USA. 2. 3Department of Epidemiology and State Key Laboratory of Oncogene and Related Genes,Shanghai Cancer Institute,Renji Hospital,Shanghai Jiaotong University School of Medicine,Shanghai,People's Republic of China. 3. 2Department of Epidemiology,School of Public Health,University of Alabama at Birmingham,Birmingham,AL,USA. 4. 4Division of Biostatistics and Epidemiology,Cincinnati Children's Hospital Medical Center,Cincinnati,OH,USA.
Abstract
OBJECTIVE: To assess correlations between cruciferous vegetable intake and urinary isothiocyanate (ITC) level, in addition to glutathione S-transferase (GST) genotypes and other individual factors. DESIGN: The study included cohort participants whose urinary ITC levels had been previously ascertained. Urinary ITC was assessed using HPLC. Usual dietary intake of cruciferous vegetables was assessed using a validated FFQ and total dietary ITC intake was calculated. Recent cruciferous vegetable intake was determined. GST genotypes were assessed using duplex real-time quantitative PCR assays. Spearman correlations were calculated between the covariates and urinary ITC levels and linear regression analyses were used to calculate the mean urinary ITC excretion according to GST genotype. SETTING: Urban city in China. SUBJECTS: The study included 3589 women and 1015 men from the Shanghai Women's and Men's Health Studies. RESULTS: Median urinary ITC level was 1.61 nmol/mg creatinine. Self-reported usual cruciferous vegetable intake was weakly correlated with urinary ITC level (r s=0.1149; P<0.0001), while self-reported recent intake was more strongly correlated with urinary ITC (r s=0.2591; P<0.0001). Overall, the GST genotypes were not associated with urinary ITC level, but significant differences according to genotype were observed among current smokers and participants who provided an afternoon urine sample. Other factors, including previous gastrectomy or gastritis, were also related to urinary ITC level. CONCLUSIONS: The study suggests that urinary secretion of ITC may provide additional information on cruciferous vegetable intake and that GST genotypes are related to urinary ITC level only in some subgroups.
OBJECTIVE: To assess correlations between cruciferous vegetable intake and urinary isothiocyanate (ITC) level, in addition to glutathione S-transferase (GST) genotypes and other individual factors. DESIGN: The study included cohort participants whose urinary ITC levels had been previously ascertained. Urinary ITC was assessed using HPLC. Usual dietary intake of cruciferous vegetables was assessed using a validated FFQ and total dietary ITC intake was calculated. Recent cruciferous vegetable intake was determined. GST genotypes were assessed using duplex real-time quantitative PCR assays. Spearman correlations were calculated between the covariates and urinary ITC levels and linear regression analyses were used to calculate the mean urinary ITC excretion according to GST genotype. SETTING: Urban city in China. SUBJECTS: The study included 3589 women and 1015 men from the Shanghai Women's and Men's Health Studies. RESULTS: Median urinary ITC level was 1.61 nmol/mg creatinine. Self-reported usual cruciferous vegetable intake was weakly correlated with urinary ITC level (r s=0.1149; P<0.0001), while self-reported recent intake was more strongly correlated with urinary ITC (r s=0.2591; P<0.0001). Overall, the GST genotypes were not associated with urinary ITC level, but significant differences according to genotype were observed among current smokers and participants who provided an afternoon urine sample. Other factors, including previous gastrectomy or gastritis, were also related to urinary ITC level. CONCLUSIONS: The study suggests that urinary secretion of ITC may provide additional information on cruciferous vegetable intake and that GST genotypes are related to urinary ITC level only in some subgroups.
Authors: Barbro Nermell; Anna-Lena Lindberg; Mahfuzar Rahman; Marika Berglund; Lars Ake Persson; Shams El Arifeen; Marie Vahter Journal: Environ Res Date: 2007-09-27 Impact factor: 6.498
Authors: S Pemble; K R Schroeder; S R Spencer; D J Meyer; E Hallier; H M Bolt; B Ketterer; J B Taylor Journal: Biochem J Date: 1994-05-15 Impact factor: 3.857