| Literature DB >> 25096719 |
Sung Eun Kim1, Young-Pil Yun1, Kyu-Sik Shim2, Kyeongsoon Park3, Sung-Wook Choi4, Dong Hun Suh5.
Abstract
The aim of this study was to develop lactoferrin (LF)-impregnated porous poly(lactide-co-glycolide) (PLGA) microspheres (PMs) to induce osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). Porous PLGA PMs were fabricated by a fluidic device and their surfaces were modified with heparin-dopamine (Hep-DOPA). Then, LF (100μg, 500μg, and 1000μg) was impregnated on the surface of heparinized PMs (Hep-PMs) via electrostatic interactions to yield LF-impregnated PMs. PMs and modified PMs were characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Osteogenic differentiation of rADSCs on PMs and modified PMs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and mRNA expression of osteocalcin and osteopontin. Successful immobilization of Hep-DOPA and LF on the surface of PMs was confirmed by XPS analysis. LF-impregnated PMs generated significantly greater ALP activity, calcium deposition, and mRNA expression of osteocalcin and osteopontin compared with PMs. These results suggested that LF-impregnated PMs effectively induced osteogenic differentiation of rADSCs.Entities:
Keywords: Fluidic device; Lactoferrin; Osteogenic differentiation; Porous microspheres; Rabbit adipose-derived stem cells
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Year: 2014 PMID： 25096719 DOI： 10.1016/j.colsurfb.2014.06.057
Source DB: PubMed Journal: Colloids Surf B Biointerfaces ISSN： 0927-7765 Impact factor: 5.268