Suyane Maria Luna Cruz de Vasconcelos1, Mary Anne Sampaio de Melo2, Joao Paulo Marques Saraiva Wenceslau3, Iriana Carla Junqueira Zanin4, Haroldo Cesar Pinheiro Beltrao5, Carlos Augusto Oliveira Fernandes5, Paulo Cesar de Almeida6, Lidiany Karla Azevedo Rodrigues7. 1. Student, Post-graduation Program in Dentistry, Faculty of Pharmacy Dentistry and Nursing, Federal University of Ceara, Ceara Brazil. 2. Assistant Professor, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore MD, USA. 3. Undergraduate Student , Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Ceara, Brazil. 4. Associate Professor, The School of Dentistry of Sobral; Professor of the Post-graduation Program in Dentistry, Federal University of Ceara Ceara, Brazil. 5. Associate Professor, Department of Operative Dentistry, Faculty of Pharmacy Dentistry and Nursing, Federal University of Ceara, Ceara Brazil. 6. Associate Professor, Department of Public Health, Ceara State University, Ceara Brazil. 7. Associate Professor, Department of Operative Dentistry, Faculty of Pharmacy Dentistry and Nursing; Professor of the Post-graduation Program in Dentistry, Federal University of Ceara, Ceara, Brazil.
Abstract
AIM: This in situ study assessed the effects of adhesive systems containing or not fluoride and/or the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the microbiological composition of dental biofilm and enamel demineralization. MATERIALS AND METHODS: During two phases of 14 days, ten volunteers wore intraoral palatal appliances containing two slabs of human enamel according to a double-blind, crossover design. The slabs were randomly restored using a composite resin and one of the following adhesive systems: All-Bond SE(TM) (self-etch, fluoride/MDPB free adhesive, AB) and Clearfl Protect Bond (self-etch containing fluoride and MDPB adhesive, CB). The biofilm formed on the slabs was analyzed with regard to total and mutans streptococci and lactobacilli counts. Demineralization represented by integrated area of hardness × lesion depth Delta S ( ΔS) was determined on enamel by analysis of cross-sectional microhardness, at 20 and 70 μm from the restoration margin. Data were analyzed by ANOVA. RESULTS: No statistically significant difference was found either in enamel demineralization or in the microbiological composition of dental biofilm. CONCLUSION: All adhesive systems containing or not fluoride and/or MDPB tested were unable to inhibit secondary caries in the in situ model used in the present research.
RCT Entities:
AIM: This in situ study assessed the effects of adhesive systems containing or not fluoride and/or the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the microbiological composition of dental biofilm and enamel demineralization. MATERIALS AND METHODS: During two phases of 14 days, ten volunteers wore intraoral palatal appliances containing two slabs of human enamel according to a double-blind, crossover design. The slabs were randomly restored using a composite resin and one of the following adhesive systems: All-Bond SE(TM) (self-etch, fluoride/MDPB free adhesive, AB) and Clearfl Protect Bond (self-etch containing fluoride and MDPB adhesive, CB). The biofilm formed on the slabs was analyzed with regard to total and mutans streptococci and lactobacilli counts. Demineralization represented by integrated area of hardness × lesion depth Delta S ( ΔS) was determined on enamel by analysis of cross-sectional microhardness, at 20 and 70 μm from the restoration margin. Data were analyzed by ANOVA. RESULTS: No statistically significant difference was found either in enamel demineralization or in the microbiological composition of dental biofilm. CONCLUSION: All adhesive systems containing or not fluoride and/or MDPB tested were unable to inhibit secondary caries in the in situ model used in the present research.
Authors: Audrey C C Hollanders; Nicolien K Kuper; Tamires T Maske; Marie-Charlotte D N J M Huysmans Journal: Caries Res Date: 2018-04-05 Impact factor: 4.056