Yanning Zhang1, Jie Wang2, Fusheng Dong3, Hexiang Li1, Yali Hou1. 1. Department of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang, PR China; The Key Laboratory of Stomatology, No. 383 East Zhongshan Road, Shijiazhuang 050017, Hebei, PR China. 2. Department of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang, PR China; The Key Laboratory of Stomatology, No. 383 East Zhongshan Road, Shijiazhuang 050017, Hebei, PR China. Electronic address: wangjiephd@163.com. 3. Department of Oral and Maxillofacial Surgery, College of Stomatology, Hebei Medical University, Shijiazhuang, PR China; The Key Laboratory of Stomatology, No. 383 East Zhongshan Road, Shijiazhuang 050017, Hebei, PR China.
Abstract
OBJECTIVE: Proteoglycans play a crucial role in salivary adenoid cystic carcinoma (SACC) tumorigenesis, neurotropic growth and lung metastasis. In this study, we investigated the role of GPC5 in the lung metastasis of SACC. DESIGN: The expression of heparan sulfate proteoglycans (HSPGs) was detected in SACC-M (high lung-metastatic cell line), SACC-2 (low lung-metastatic cell line) and SACC-83 (low lung-metastatic cell line) cells by relative quantitative Real-Time PCR. The expression of GPC5 was detected by immunofluorescence and Western blot analysis in SACC-M, SACC-2 and SACC-83 cells, and by immunohistochemical analysis in primary tumours from 16 cases of SACC patients with or without lung metastasis. GPC5 expression was silenced in SACC-M cells, cell proliferation and tumour growth was evaluated by MTT assay and nude mice model, respectively. RESULTS: Expression of most HSPGs was decreased in SACC-M cells, but GPC5 expression increased 3.24-fold and 815.69-fold (more than 3-fold) in SACC-M compared with SACC-2 and SACC-83 cells, respectively. Immunohistochemical analysis showed higher expression of GPC5 in SACC with lung metastasis compared to SACC without lung metastasis. The lung metastasis of SACC-M cells in nude mice was obviously decreased after GPC5 silencing (P<0.05), although GPC5 silencing had no significant effect on SACC-M cell proliferation. CONCLUSION: GPC5 may contribute to lung metastasis of SACC.
OBJECTIVE: Proteoglycans play a crucial role in salivary adenoid cystic carcinoma (SACC) tumorigenesis, neurotropic growth and lung metastasis. In this study, we investigated the role of GPC5 in the lung metastasis of SACC. DESIGN: The expression of heparan sulfate proteoglycans (HSPGs) was detected in SACC-M (high lung-metastatic cell line), SACC-2 (low lung-metastatic cell line) and SACC-83 (low lung-metastatic cell line) cells by relative quantitative Real-Time PCR. The expression of GPC5 was detected by immunofluorescence and Western blot analysis in SACC-M, SACC-2 and SACC-83 cells, and by immunohistochemical analysis in primary tumours from 16 cases of SACC patients with or without lung metastasis. GPC5 expression was silenced in SACC-M cells, cell proliferation and tumour growth was evaluated by MTT assay and nude mice model, respectively. RESULTS: Expression of most HSPGs was decreased in SACC-M cells, but GPC5 expression increased 3.24-fold and 815.69-fold (more than 3-fold) in SACC-M compared with SACC-2 and SACC-83 cells, respectively. Immunohistochemical analysis showed higher expression of GPC5 in SACC with lung metastasis compared to SACC without lung metastasis. The lung metastasis of SACC-M cells in nude mice was obviously decreased after GPC5 silencing (P<0.05), although GPC5 silencing had no significant effect on SACC-M cell proliferation. CONCLUSION:GPC5 may contribute to lung metastasis of SACC.