OBJECTIVE: Innate immune protein C1q plays a dual role in the chronic inflammatory disease of atherosclerosis. Complement activation via C1q exacerbates pathology in the atherosclerotic lesion in later stages of the disease. However, in early stages of disease C1q is protective. We hypothesize that complement-independent activities of C1q are involved in reprogramming macrophage inflammatory polarization. METHODS: The influence of C1q on macrophage inflammatory responses during clearance of oxLDL was examined. Changes in cytokines at the gene and protein level were measured by quantitative PCR and ELISA assay. RESULTS: C1q modulated cytokine expression in Raw264.7 macrophages during ingestion of oxLDL. Levels of pro-inflammatory cytokines IL-1β and IL-6 were downregulated by C1q, whereas levels of the anti-inflammatory cytokine IL-10 were increased. In addition, data from an NFκB-luciferase gene reporter assay suggest that C1q suppresses activation of NFκB during lipoprotein clearance in macrophages, providing one mechanism by which C1q downregulates pro-inflammatory cytokine production. CONCLUSIONS: C1q-polarization of macrophages toward an anti-inflammatory (M2-like) phenotype may be important in dampening inflammation in the early atherosclerotic lesion. Further investigation of molecular pathways targeted by C1q may provide novel therapeutic targets for this disease.
OBJECTIVE: Innate immune protein C1q plays a dual role in the chronic inflammatory disease of atherosclerosis. Complement activation via C1q exacerbates pathology in the atherosclerotic lesion in later stages of the disease. However, in early stages of disease C1q is protective. We hypothesize that complement-independent activities of C1q are involved in reprogramming macrophage inflammatory polarization. METHODS: The influence of C1q on macrophage inflammatory responses during clearance of oxLDL was examined. Changes in cytokines at the gene and protein level were measured by quantitative PCR and ELISA assay. RESULTS:C1q modulated cytokine expression in Raw264.7 macrophages during ingestion of oxLDL. Levels of pro-inflammatory cytokines IL-1β and IL-6 were downregulated by C1q, whereas levels of the anti-inflammatory cytokine IL-10 were increased. In addition, data from an NFκB-luciferase gene reporter assay suggest that C1q suppresses activation of NFκB during lipoprotein clearance in macrophages, providing one mechanism by which C1q downregulates pro-inflammatory cytokine production. CONCLUSIONS:C1q-polarization of macrophages toward an anti-inflammatory (M2-like) phenotype may be important in dampening inflammation in the early atherosclerotic lesion. Further investigation of molecular pathways targeted by C1q may provide novel therapeutic targets for this disease.
Authors: J Lauran Stöger; Marion J J Gijbels; Saskia van der Velden; Marco Manca; Chris M van der Loos; Erik A L Biessen; Mat J A P Daemen; Esther Lutgens; Menno P J de Winther Journal: Atherosclerosis Date: 2012-09-26 Impact factor: 5.162
Authors: M Botto; C Dell'Agnola; A E Bygrave; E M Thompson; H T Cook; F Petry; M Loos; P P Pandolfi; M J Walport Journal: Nat Genet Date: 1998-05 Impact factor: 38.330
Authors: Sonia I Vlaicu; Alexandru Tatomir; Violeta Rus; Armugam P Mekala; Petru A Mircea; Florin Niculescu; Horea Rus Journal: Immunol Res Date: 2016-02 Impact factor: 2.829
Authors: C A Gomez-Roca; A Italiano; C Le Tourneau; P A Cassier; M Toulmonde; S P D'Angelo; M Campone; K L Weber; D Loirat; M A Cannarile; A-M Jegg; C Ries; R Christen; G Meneses-Lorente; W Jacob; I Klaman; C-H Ooi; C Watson; K Wonde; B Reis; F Michielin; D Rüttinger; J-P Delord; J-Y Blay Journal: Ann Oncol Date: 2019-08-01 Impact factor: 32.976
Authors: Shengjie Xu-Vanpala; M Elizabeth Deerhake; Joshua D Wheaton; Morgan E Parker; Praveen R Juvvadi; Nancie MacIver; Maria Ciofani; Mari L Shinohara Journal: Sci Immunol Date: 2020-08-07