| Literature DB >> 25089958 |
Peter Podolec1, Alexandra Hengerics Szabó1, Jaroslav Blaško1, Róbert Kubinec2, Renáta Górová1, Jozef Višňovský3, Anna Gnipová4, Anton Horváth4, Václav Bierhanzl5, Tomáš Hložek6, Radomír Čabala7.
Abstract
A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mg L(-1) for glucose to 0.85 mg L(-1) for lactate.Entities:
Keywords: Aqueous samples; GC–MS/MS analysis; Metabolites; Protozoa; Trimethylsilylation
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Year: 2014 PMID： 25089958 DOI： 10.1016/j.jchromb.2014.07.023
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205