| Literature DB >> 25086674 |
Guoxin Mao1, Liting Lv1, Yifei Liu2, Buyou Chen1, Mei Li1, Tingting Ni1, Dunpeng Yang3, Hongzhen Zhu1, Qun Xue4, Runzhou Ni5.
Abstract
High temperature required A2 (HtrA2) is a serine kinase that is released from mitochondria into the cytosol upon apoptotic stimuli, inducing apoptosis in various cancers. Thus, analysis of the expression of HtrA2 in non-small-cell lung cancer (NSCLC) tissues is needed for the understanding of this malignancy. In this study we firstly analyzed the apoptosis effect of HtrA2 in A549 cells by RNA interference and cisplatin with Western blot and flow cytometry. Then HtrA2 expression was evaluated by Western blot and immunohistochemistry in NSCLC tissues. Western blot and flow cytometry analyses indicated that deletion of HtrA2 was negatively correlated with apoptosis-induced protein in A549 cells. HtrA2 was lowly expressed in NSCLC and significantly associated with histological differentiation and clinical stage. Besides, low expression of HtrA2 was a prognostic factor for NSCLC patients' inferior survival. In conclusion, HtrA2 might promote the apoptosis of NSCLC cells, and serve as a target for NSCLC's treatment.Entities:
Keywords: Apoptosis; HtrA2; Non-small-cell lung cancer; Prognosis
Mesh：
Substances：
Year: 2014 PMID： 25086674 DOI： 10.1016/j.prp.2014.06.030
Source DB: PubMed Journal: Pathol Res Pract ISSN： 0344-0338 Impact factor: 3.250