Literature DB >> 25086024

Purification and culture of dorsal root ganglion neurons.

J Bradley Zuchero1.   

Abstract

Dorsal root ganglion neurons (DRGs) are sensory neurons that reside in ganglions on the dorsal root of the spinal cord. Here we introduce a method for the acute, prospective purification and culture of DRGs from rodents in a serum-free, defined medium, in the absence of glial cells. This immunopanning-based method facilitates the study of DRG biology and function.
© 2014 Cold Spring Harbor Laboratory Press.

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Year:  2014        PMID: 25086024      PMCID: PMC4438772          DOI: 10.1101/pdb.top073965

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN: 1559-6095


  28 in total

1.  The tetraspanin protein, CD9, is expressed by progenitor cells committed to oligodendrogenesis and is linked to beta1 integrin, CD81, and Tspan-2.

Authors:  Nobuo Terada; Karen Baracskay; Mike Kinter; Shona Melrose; Peter J Brophy; Claude Boucheix; Carl Bjartmar; Grahame Kidd; Bruce D Trapp
Journal:  Glia       Date:  2002-12       Impact factor: 7.452

2.  A novel method of eliminating non-neuronal proliferating cells from cultures of mouse dorsal root ganglia.

Authors:  Parker L Andersen; J Ronald Doucette; Adil J Nazarali
Journal:  Cell Mol Neurobiol       Date:  2003-04       Impact factor: 5.046

3.  NGF controls axonal receptivity to myelination by Schwann cells or oligodendrocytes.

Authors:  Jonah R Chan; Trent A Watkins; José M Cosgaya; ChunZhao Zhang; Lian Chen; Louis F Reichardt; Eric M Shooter; Ben A Barres
Journal:  Neuron       Date:  2004-07-22       Impact factor: 17.173

4.  Adult mouse dorsal root ganglia neurons in cell culture.

Authors:  B S Scott
Journal:  J Neurobiol       Date:  1977-09

5.  Separation of functional Schwann cells and neurons from normal peripheral nerve tissue.

Authors:  P M Wood
Journal:  Brain Res       Date:  1976-10-22       Impact factor: 3.252

6.  Monoclonal antibodies against carbohydrate differentiation antigens identify subsets of primary sensory neurones.

Authors:  J Dodd; D Solter; T M Jessell
Journal:  Nature       Date:  1984 Oct 4-10       Impact factor: 49.962

7.  Pure population of viable neurons from rabbit dorsal root ganglia, using gradients of Percoll.

Authors:  S S Goldenberg; U De Boni
Journal:  J Neurobiol       Date:  1983-05

8.  The WldS protein protects against axonal degeneration: a model of gene therapy for peripheral neuropathy.

Authors:  M S Wang; G Fang; D G Culver; A A Davis; M M Rich; J D Glass
Journal:  Ann Neurol       Date:  2001-12       Impact factor: 10.422

9.  The use of networks of dissociated rat dorsal root ganglion neurons to induce myelination by oligodencrocytes in culture.

Authors:  P Wood; E Okada; R Bunge
Journal:  Brain Res       Date:  1980-08-25       Impact factor: 3.252

10.  Studies of Schwann cell proliferation. I. An analysis in tissue culture of proliferation during development, Wallerian degeneration, and direct injury.

Authors:  J L Salzer; R P Bunge
Journal:  J Cell Biol       Date:  1980-03       Impact factor: 10.539

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  4 in total

1.  Cold shock induces apoptosis of dorsal root ganglion neurons plated on infrared windows.

Authors:  Ebrahim Aboualizadeh; Eric C Mattson; Crystal L O'Hara; Amanda K Smith; Cheryl L Stucky; Carol J Hirschmugl
Journal:  Analyst       Date:  2015-05-22       Impact factor: 4.616

2.  Purification of dorsal root ganglion neurons from rat by immunopanning.

Authors:  J Bradley Zuchero
Journal:  Cold Spring Harb Protoc       Date:  2014-08-01

3.  Three-Dimensional Model of Dorsal Root Ganglion Explant as a Method of Studying Neurotrophic Factors in Regenerative Medicine.

Authors:  Polina Klimovich; Kseniya Rubina; Veronika Sysoeva; Ekaterina Semina
Journal:  Biomedicines       Date:  2020-03-03

4.  Constitutional mislocalization of Pten drives precocious maturation in oligodendrocytes and aberrant myelination in model of autism spectrum disorder.

Authors:  Hyunpil Lee; Stetson Thacker; Nicholas Sarn; Ranjan Dutta; Charis Eng
Journal:  Transl Psychiatry       Date:  2019-01-17       Impact factor: 6.222

  4 in total

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