| Literature DB >> 25084654 |
U Aksoy1, M Marangi2, R Papini3, S Ozkoc1, S Bayram Delibas1, A Giangaspero4.
Abstract
To evaluate the presence of Toxoplasma gondii and Cyclospora cayetanensis in edible shellfish, a total of 795 specimens of Mytilus galloprovincialis from 8 different sites on the west coast of Turkey (Bays of Izmir and Mersin) were examined. Fifty-three pooled samples were created and subjected to EvaGreen(®) RealTime PCR assay and HRM analysis for protozoans detection and oocyst quantification. Seven out of 8 sites were contaminated with at least one protozoan species. Twenty-one out of 53 (39.6%) samples tested positive for T. gondii, C. cayetanensis or both pathogens: 26.4% samples positive for C. cayetanensis; 9.4% for T. gondii, and 3.8% for both protozoans. The number of oocysts in positive samples ranged from 6 to 30 for T. gondii and from 3 to 48 for C. cayetanensis. This is the first report of T. gondii Type 1, and C. cayetanensis in M. galloprovincialis, the most consumed shellfish species in Turkey.Entities:
Keywords: Cyclospora cayetanensis; Mytilus galloprovincialis; Public health; RealTime HRM; Toxoplasma gondii Type I
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Year: 2014 PMID： 25084654 DOI： 10.1016/j.fm.2014.05.012
Source DB: PubMed Journal: Food Microbiol ISSN： 0740-0020 Impact factor: 5.516