Literature DB >> 25081741

Thermodynamic analysis of weak protein interactions using sedimentation equilibrium.

Yuri V Sergeev1, Monika B Dolinska1, Paul T Wingfield2.   

Abstract

Proteins self-associate to form dimers and tetramers. Purified proteins are used to study the thermodynamics of protein interactions using the analytical ultracentrifuge. In this approach, monomer-dimer equilibrium constants are directly measured at various temperatures. Data analysis is used to derive thermodynamic parameters, such as Gibbs free energy, enthalpy, and entropy, which can predict which major forces are involved in protein association.
Copyright © 2014 John Wiley & Sons, Inc.

Entities:  

Keywords:  sedimentation equilibrium; thermodynamics; weak protein interaction

Mesh：

Substances：

Year:  2014        PMID： 25081741      PMCID： PMC4182932          DOI： 10.1002/0471140864.ps2013s77

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  17 in total

1.  Monomer-dimer equilibrium of normal and modified beta A3-crystallins: experimental determination and molecular modeling.

Authors:  Y V Sergeev; P T Wingfield; J F Hejtmancik
Journal:  Biochemistry       Date:  2000-12-26       Impact factor: 3.162

2.  Deamidation of human beta B1 alters the elongated structure of the dimer.

Authors:  K J Lampi; J T Oxford; H P Bachinger; T R Shearer; L L David; D M Kapfer
Journal:  Exp Eye Res       Date:  2001-03       Impact factor: 3.467

3.  Energetics of domain-domain interactions and entropy driven association of beta-crystallins.

Authors:  Y V Sergeev; J F Hejtmancik; P T Wingfield
Journal:  Biochemistry       Date:  2004-01-20       Impact factor: 3.162

4.  Direct spectrophotometric measurement of the rate of reduction of disulfide bonds. The reactivity of the disulfide bonds of bovine -lactalbumin.

Authors:  K S Iyer; W A Klee
Journal:  J Biol Chem       Date:  1973-01-25       Impact factor: 5.157

5.  Thermodynamics of protein association reactions: forces contributing to stability.

Authors:  P D Ross; S Subramanian
Journal:  Biochemistry       Date:  1981-05-26       Impact factor: 3.162

6.  Association properties of betaB1- and betaA3-crystallins: ability to form heterotetramers.

Authors:  May P Chan; Monika Dolinska; Yuri V Sergeev; Paul T Wingfield; J Fielding Hejtmancik
Journal:  Biochemistry       Date:  2008-09-30       Impact factor: 3.162

7.  X-ray analysis of the eye lens protein gamma-II crystallin at 1.9 A resolution.

Authors:  G Wistow; B Turnell; L Summers; C Slingsby; D Moss; L Miller; P Lindley; T Blundell
Journal:  J Mol Biol       Date:  1983-10-15       Impact factor: 5.469

8.  N-terminal extension of beta B1-crystallin: identification of a critical region that modulates protein interaction with beta A3-crystallin.

Authors:  Monika B Dolinska; Yuri V Sergeev; May P Chan; Ira Palmer; Paul T Wingfield
Journal:  Biochemistry       Date:  2009-10-13       Impact factor: 3.162

9.  βB1-crystallin: thermodynamic profiles of molecular interactions.

Authors:  Monika B Dolinska; Paul T Wingfield; Yuri V Sergeev
Journal:  PLoS One       Date:  2012-01-06       Impact factor: 3.240

10.  Deamidation alters interactions of beta-crystallins in hetero-oligomers.

Authors:  Takumi Takata; Luke G Woodbury; Kirsten J Lampi
Journal:  Mol Vis       Date:  2009-01-28       Impact factor: 2.367
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  2 in total

1.  Enthalpy-Driven Stabilization of Transthyretin by AG10 Mimics a Naturally Occurring Genetic Variant That Protects from Transthyretin Amyloidosis.

Authors:  Mark Miller; Arindom Pal; Wabel Albusairi; Hyun Joo; Beverly Pappas; Md Tariqul Haque Tuhin; Dengpan Liang; Raghavendra Jampala; Fang Liu; Jared Khan; Marjon Faaij; Miki Park; William Chan; Isabella Graef; Robert Zamboni; Neil Kumar; Jonathan Fox; Uma Sinha; Mamoun Alhamadsheh
Journal:  J Med Chem       Date:  2018-08-22       Impact factor: 7.446

2.  Human Tyrosinase: Temperature-Dependent Kinetics of Oxidase Activity.

Authors:  Kenneth L Young; Claudia Kassouf; Monika B Dolinska; David Eric Anderson; Yuri V Sergeev
Journal:  Int J Mol Sci       Date:  2020-01-30       Impact factor: 5.923
  2 in total

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