Literature DB >> 25081

On the measurement of pH in Escherichia coli by 31P nuclear magnetic resonance.

S Ogawa, R G Shulman, P Glynn, T Yamane, G Navon.   

Abstract

The 31P high resolution NMR spectra of concentrated suspensions of Escherichia coli cells have been measured at 145.8 MHz. The position of the orthophosphate resonance is used as a measure of internal and external pH. In accord with Paddan, Zilberstein and Rottenberg ((1976) Eur. J. Biochem. 63, 533--541) it is shown that when properly energized the internal pH is 7.5 +/- 0.1. By synchronizing the NMR data acquisition with 3-s bursts of O2 it is possible to measure the internal pH with a time resolution of about 1 s. It is shown that at 20 degrees C the pH remains constant for times longer than 15 s after the oxygen is discontinued and it decays in several minutes.

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Year:  1978        PMID： 25081     DOI： 10.1016/0005-2728(78)90130-5

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  10 in total

1.  Control of Escherichia coli lysyl-tRNA synthetase expression by anaerobiosis.

Authors:  F Lévêque; M Gazeau; M Fromant; S Blanquet; P Plateau
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490

2.  Measurement of an intracellular pH rise after fertilization in crab eggs using 31P-NMR.

Authors:  M Hervé; M Goudeau; J M Neumann; J C Debouzy; H Goudeau
Journal:  Eur Biophys J       Date:  1989       Impact factor: 1.733

3.  31P nuclear magnetic resonance studies of effects of some chlorophenols on Escherichia coli and a pentachlorophenol-degrading bacterium.

Authors:  J G Steiert; W J Thoma; K Ugurbil; R L Crawford
Journal:  J Bacteriol       Date:  1988-10       Impact factor: 3.490

Review 4.  Development of functional imaging in the human brain (fMRI); the University of Minnesota experience.

Authors:  Kâmil Uğurbil
Journal:  Neuroimage       Date:  2012-02-08       Impact factor: 6.556

5.  Phosphorylation status of liver by 31P-n.m.r. spectroscopy, and its implications for metabolic control. A comparison of 31P-n.m.r. spectroscopy (in vivo and in vitro) with chemical and enzymic determinations of ATP, ADP and Pi.

Authors:  R A Iles; A N Stevens; J R Griffiths; P G Morris
Journal:  Biochem J       Date:  1985-07-01       Impact factor: 3.857

6.  Electrochemical proton gradient in Micrococcus lysodeikticus cells and membrane vesicles.

Authors:  I Friedberg; H R Kaback
Journal:  J Bacteriol       Date:  1980-05       Impact factor: 3.490

7.  13C NMR studies of gluconeogenesis in rat liver cells: utilization of labeled glycerol by cells from euthyroid and hyperthyroid rats.

Authors:  S M Cohen; S Ogawa; R G Shulman
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

8.  Analysis of rat heart in vivo by phosphorus nuclear magnetic resonance.

Authors:  T H Grove; J J Ackerman; G K Radda; P J Bore
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

9.  Measurement of changes in high-energy phosphates in the cardiac cycle using gated 31P nuclear magnetic renonance.

Authors:  E T Fossel; H E Morgan; J S Ingwall
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

10.  pH homeostasis in Escherichia coli: measurement by 31P nuclear magnetic resonance of methylphosphonate and phosphate.

Authors:  J L Slonczewski; B P Rosen; J R Alger; R M Macnab
Journal:  Proc Natl Acad Sci U S A       Date:  1981-10       Impact factor: 11.205
  10 in total

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