Z Maghbooli1, A Hossein-Nezhad, B Larijani, P Pasalar, A A Keshtkar. 1. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism,Clinical Sciences Institute, Tehran, University of Medical Sciences, Tehran, Iran - arash_hsi@yahoo.com.
Abstract
AIM: The aim of this study was to investigate the relationship between inter-individual global DNA methylation and diabetes predisposing factors. METHODS: The 5-methyl cytosine content was assessed by reverse phase high pressure liquid chromatography (RP-HPLC) of peripheral blood leukocytes obtained from 178 type 2 diabetes patients to determine individual global DNA methylation status. RESULTS: There was a positive significant correlation between diabetes duration and DNA methylation levels (P=0.002) with increasing levels of DNA methylation associated with age (P=0.047). There was no significant correlation between DNA methylation levels and HbA1c (P=0.15). No significant differences were observed between patients with and without diabetes predisposing factors including: hypertension (P=0.772), dyslipidemia (P=0.617), insulin resistance (homeostatic model assessment index) (P=0.156) and obesity (P=0.609). As such, the duration of diabetes (>10 years) was the most important predictor of global DNA methylation levels in diabetic patients after adjusting for age and sex (P=0.023). CONCLUSION: Our findings indicate that chronic hyperglycemic exposure plays an independent role in global DNA methylation levels in type 2 diabetes patients.
AIM: The aim of this study was to investigate the relationship between inter-individual global DNA methylation and diabetes predisposing factors. METHODS: The 5-methyl cytosine content was assessed by reverse phase high pressure liquid chromatography (RP-HPLC) of peripheral blood leukocytes obtained from 178 type 2 diabetespatients to determine individual global DNA methylation status. RESULTS: There was a positive significant correlation between diabetes duration and DNA methylation levels (P=0.002) with increasing levels of DNA methylation associated with age (P=0.047). There was no significant correlation between DNA methylation levels and HbA1c (P=0.15). No significant differences were observed between patients with and without diabetes predisposing factors including: hypertension (P=0.772), dyslipidemia (P=0.617), insulin resistance (homeostatic model assessment index) (P=0.156) and obesity (P=0.609). As such, the duration of diabetes (>10 years) was the most important predictor of global DNA methylation levels in diabeticpatients after adjusting for age and sex (P=0.023). CONCLUSION: Our findings indicate that chronic hyperglycemic exposure plays an independent role in global DNA methylation levels in type 2 diabetespatients.
Authors: C Reichetzeder; S E Dwi Putra; B Kleuser; B Hocher; T Pfab; T Slowinski; C Neuber Journal: Clin Epigenetics Date: 2016-07-26 Impact factor: 6.551