Literature DB >> 2507722

Purification of glycerol dialkyl nonitol tetraether from Sulfolobus acidocaldarius.

S L Lo1, C E Montague, E L Chang.   

Abstract

A modified procedure for extraction and purification of hydrolyzed archaebacterial lipids is described. Lipids were extracted from Sulfolobus acidocaldarius using a Soxhlet extraction procedure followed by trichloroacetic acid solvent-extraction of the residue. The yield of total extractable material by this protocol was 14% which, after a two-phase wash, yielded 10% lipid. Modifications to the published steps for purifying the subsequently hydrolyzed lipids were developed to purify glycerol dialkyl nonitol tetraether (GDNT). The nearly colorless final macrocyclic product was characterized by TLC, IR, NMR, and mass spectrometry.

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Year:  1989        PMID： 2507722

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  6 in total

1.  Archaeal lipids and their biotechnological applications.

Authors:  A Gambacorta; A Gliozzi; M De Rosa
Journal:  World J Microbiol Biotechnol       Date:  1995-01       Impact factor: 3.312

2.  Compressibilities and volume fluctuations of archaeal tetraether liposomes.

Authors:  Parkson Lee-Gau Chong; Michael Sulc; Roland Winter
Journal:  Biophys J       Date:  2010-11-17       Impact factor: 4.033

3.  The structure of the core polyol of the ether lipids from Sulfolobus acidocaldarius.

Authors:  A Sugai; R Sakuma; I Fukuda; N Kurosawa; Y H Itoh; K Kon; S Ando; T Itoh
Journal:  Lipids       Date:  1995-04       Impact factor: 1.880

4.  Sulfolobus acidocaldarius Microvesicles Exhibit Unusually Tight Packing Properties as Revealed by Optical Spectroscopy.

Authors:  Alexander Bonanno; Robert C Blake; Parkson Lee-Gau Chong
Journal:  Int J Mol Sci       Date:  2019-10-25       Impact factor: 5.923

5.  Polar Lipid Fraction E from Sulfolobus acidocaldarius and Dipalmitoylphosphatidylcholine Can Form Stable yet Thermo-Sensitive Tetraether/Diester Hybrid Archaeosomes with Controlled Release Capability.

Authors:  Umme Ayesa; Parkson Lee-Gau Chong
Journal:  Int J Mol Sci       Date:  2020-11-09       Impact factor: 5.923

6.  Certain, but Not All, Tetraether Lipids from the Thermoacidophilic Archaeon Sulfolobus acidocaldarius Can Form Black Lipid Membranes with Remarkable Stability and Exhibiting Mthk Channel Activity with Unusually High Ca2+ Sensitivity.

Authors:  Alexander Bonanno; Parkson Lee-Gau Chong
Journal:  Int J Mol Sci       Date:  2021-11-30       Impact factor: 5.923
  6 in total

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