Tomoko Inoue1, Anthony Swain2, Yoichi Nakanishi3, Daisuke Sugiyama1. 1. Department of Research and Development of Next-Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan yokotomo@hsc.med.kyushu-u.ac.jp ds-mons@yb3.so-net.ne.jp. 2. Department of Research and Development of Next-Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan. 3. Center for Clinical and Translational Research, Kyushu University Hospital, Fukuoka, Japan.
Abstract
BACKGROUND: Leukemia cell lines are utilized as tools for molecular analysis. Their implementation in therapy will require standards for quality control, including appropriate selection criteria for functional analysis and efficacy determination. MATERIALS AND METHODS: Characteristics of six human leukemia cell lines -Kasumi-1, NB-4, MOLM-13, MV-4-11, K562, and Jurkat cells-were investigated using multiple color analysis of surface antigen expression and comparative analysis of gene expression. RESULTS: Differentiation states of Kasumi-1 and MOLM-13 cells are colony-forming units-granulocyte/macrophage equivalent cells to myeloblasts with comparatively high Growth factor independent-1(GFI1) and Transcription factor PU.1 (PU.1) expression, respectively. NB4 and MV-4-11 express high levels of CCAAT/enhancer-binding protein-alpha (CEBPα) and differentiate from myeloblasts to pro-monocytes and myeloblasts, respectively. K562 cells are colony-forming units-erythroid equivalent cells to erythroblasts, with the highest expression of GATA-binding factor 2 (GATA2), GATA1 and Friend of gata-1 (FOG1). Jurkat cells are pro-T to mature T-cells with the highest Neurogenic locus notch-1 homolog protein 1 (NOTCH1) expression. CONCLUSION: Our study gives a useful guideline of standards for appropriate usage of leukemia cell lines for examining novel targets in vitro. Copyright
BACKGROUND:Leukemia cell lines are utilized as tools for molecular analysis. Their implementation in therapy will require standards for quality control, including appropriate selection criteria for functional analysis and efficacy determination. MATERIALS AND METHODS: Characteristics of six humanleukemia cell lines -Kasumi-1, NB-4, MOLM-13, MV-4-11, K562, and Jurkat cells-were investigated using multiple color analysis of surface antigen expression and comparative analysis of gene expression. RESULTS: Differentiation states of Kasumi-1 and MOLM-13 cells are colony-forming units-granulocyte/macrophage equivalent cells to myeloblasts with comparatively high Growth factor independent-1(GFI1) and Transcription factor PU.1 (PU.1) expression, respectively. NB4 and MV-4-11 express high levels of CCAAT/enhancer-binding protein-alpha (CEBPα) and differentiate from myeloblasts to pro-monocytes and myeloblasts, respectively. K562 cells are colony-forming units-erythroid equivalent cells to erythroblasts, with the highest expression of GATA-binding factor 2 (GATA2), GATA1 and Friend of gata-1 (FOG1). Jurkat cells are pro-T to mature T-cells with the highest Neurogenic locus notch-1 homolog protein 1 (NOTCH1) expression. CONCLUSION: Our study gives a useful guideline of standards for appropriate usage of leukemia cell lines for examining novel targets in vitro. Copyright
Authors: Yen K Lieu; Zhaoqi Liu; Abdullah M Ali; Xin Wei; Alex Penson; Jian Zhang; Xiuli An; Raul Rabadan; Azra Raza; James L Manley; Siddhartha Mukherjee Journal: Proc Natl Acad Sci U S A Date: 2022-01-04 Impact factor: 12.779
Authors: Margaret Am Nelson; Kelsey L McLaughlin; James T Hagen; Hannah S Coalson; Cameron Schmidt; Miki Kassai; Kimberly A Kew; Joseph M McClung; P Darrell Neufer; Patricia Brophy; Nasreen A Vohra; Darla Liles; Myles C Cabot; Kelsey H Fisher-Wellman Journal: Elife Date: 2021-06-16 Impact factor: 8.140