Shigeo Koido1, Yutaka Enomoto2, Vasso Apostolopoulos3, Jianlin Gong4. 1. Department of Internal Medicine, The Jikei University School of Medicine, Chiba, Japan shigeo_koido@jikei.ac.jp jgong@bu.edu. 2. Department of Urology, Mitsui Memorial Hospital, Tokyo, Japan. 3. College of Health and Biomedicine, Centre for Chronic Disease Prevention and Management, Victoria University, Melbourne, Australia. 4. Department of Medicine, Boston University School of Medicine, Boston, MA, U.S.A. shigeo_koido@jikei.ac.jp jgong@bu.edu.
Abstract
BACKGROUND/AIM: Vaccination with fusions of dendritic cells (DCs) and mucin-1 (MUC1)-positive tumor cells (FC/MUC1) induces MUC1-specific antitumor immunity. However, little is known about the function of Cluster of Differentiation (CD)4 T-cells primed with FC/MUC1 in MUC1 transgenic (MUC1.Tg) mice. MATERIALS AND METHODS: CD4 T-cells primed with FC/MUC1 were analyzed by flow cytometry. Antitumor immunity by adoptive transfer of primed CD4 T-cells in Rag2(-/-) mice was assessed. RESULTS: The effector and memory T-cells generated with FC/MUC1 were crucial to maintenance of long-term antitumor immunity. MUC1-8-mer peptide SAPDTRPA presented by FC/MUC1 was recognized by CD4 and CD8 T-cells. A subset of primed CD4 T-cells possessed cytotoxicity to lyse major histocompatibility complex (MHC) class I and MUC1 positive tumor cells. Interestingly, adoptive transfer of primed CD4 T-cells prevented lung metastasis in Rag2(-/-) mice. CONCLUSION: CD4 T-cells primed by FC/MUC1 play direct role in antitumor immunity. Copyright
BACKGROUND/AIM: Vaccination with fusions of dendritic cells (DCs) and mucin-1 (MUC1)-positive tumor cells (FC/MUC1) induces MUC1-specific antitumor immunity. However, little is known about the function of Cluster of Differentiation (CD)4 T-cells primed with FC/MUC1 in MUC1 transgenic (MUC1.Tg) mice. MATERIALS AND METHODS:CD4 T-cells primed with FC/MUC1 were analyzed by flow cytometry. Antitumor immunity by adoptive transfer of primed CD4 T-cells in Rag2(-/-) mice was assessed. RESULTS: The effector and memory T-cells generated with FC/MUC1 were crucial to maintenance of long-term antitumor immunity. MUC1-8-mer peptide SAPDTRPA presented by FC/MUC1 was recognized by CD4 and CD8 T-cells. A subset of primed CD4 T-cells possessed cytotoxicity to lyse major histocompatibility complex (MHC) class I and MUC1 positive tumor cells. Interestingly, adoptive transfer of primed CD4 T-cells prevented lung metastasis in Rag2(-/-) mice. CONCLUSION:CD4 T-cells primed by FC/MUC1 play direct role in antitumor immunity. Copyright
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