AIM: To establish an orthotopic mouse model of pancreatic cancer that mimics the pathological features of exocrine pancreatic adenocarcinoma. METHODS: Pan02 cells were suspended in low-temperature Matrigel and injected into the parenchyma of pancreatic tails of C57BL/6 mice, with cells suspended in phosphate buffered saline (PBS) serving as a control. Primary and implanted tumors were confirmed pathologically. The rate of tumor formation and intraperitoneal implantation in the two groups were compared at different time points after injection. Leakage and intra-abdominal dispersion of Matrigel and PBS, both dyed with methylene blue, were compared after injection into the parenchyma of the pancreas. We observed adherence and proliferation in Pan02 cells suspended in Matrigel in vitro. We also compared the pathological manifestation of this orthotopic pancreatic cancer model in the head and tails of the pancreas. The characteristics of the origin of epithelial cells and exocrine markers of established orthotopic pancreatic tumors were confirmed using immunohistochemistry. RESULTS: Diluted Matrigel could form a gel drip in the pancreatic parenchyma, effectively preventing leakage from the injection site and avoiding dispersion in the abdominal cavity. Pan02 cells were able to adhere to a dish, proliferate, and migrate in the gel drip. The tumor formation rate in the Matrigel group was 100% at both 2 and 3 wk after injection, whereas it was 25.0% and 37.5% in the PBS group at 2 and 3 wk, respectively (P < 0.05). The intraperitoneal tumor implantation rate was 75.0% in the PBS group after 3 wk of injection, while it was 12.5% in the Matrigel group (P < 0.05). Hepatoduodenal ligament and duodenal invasions with obstructive jaundice and upper digestive obstruction with mesenteric lymph node metastasis were observed in the pancreatic head group. In the pancreatic tail group, spleen and gastric invasion were dominant, leading to retroperitoneal lymph nodes metastasis. Positive immunohistochemical staining of cytokeratin and negative staining of vimentin and chromogranin A confirmed that the orthotopic pancreatic tumor injected with Pan02 cells suspended in Matrigel was of epithelial origin and expressed exocrine markers of cancer. CONCLUSION: This method of low-temperature Matrigel suspension and injection is effective for establishing an orthotopic mouse model of pancreatic cancer.
AIM: To establish an orthotopic mouse model of pancreatic cancer that mimics the pathological features of exocrine pancreatic adenocarcinoma. METHODS: Pan02 cells were suspended in low-temperature Matrigel and injected into the parenchyma of pancreatic tails of C57BL/6 mice, with cells suspended in phosphate buffered saline (PBS) serving as a control. Primary and implanted tumors were confirmed pathologically. The rate of tumor formation and intraperitoneal implantation in the two groups were compared at different time points after injection. Leakage and intra-abdominal dispersion of Matrigel and PBS, both dyed with methylene blue, were compared after injection into the parenchyma of the pancreas. We observed adherence and proliferation in Pan02 cells suspended in Matrigel in vitro. We also compared the pathological manifestation of this orthotopic pancreatic cancer model in the head and tails of the pancreas. The characteristics of the origin of epithelial cells and exocrine markers of established orthotopic pancreatic tumors were confirmed using immunohistochemistry. RESULTS: Diluted Matrigel could form a gel drip in the pancreatic parenchyma, effectively preventing leakage from the injection site and avoiding dispersion in the abdominal cavity. Pan02 cells were able to adhere to a dish, proliferate, and migrate in the gel drip. The tumor formation rate in the Matrigel group was 100% at both 2 and 3 wk after injection, whereas it was 25.0% and 37.5% in the PBS group at 2 and 3 wk, respectively (P < 0.05). The intraperitoneal tumor implantation rate was 75.0% in the PBS group after 3 wk of injection, while it was 12.5% in the Matrigel group (P < 0.05). Hepatoduodenal ligament and duodenal invasions with obstructive jaundice and upper digestive obstruction with mesenteric lymph node metastasis were observed in the pancreatic head group. In the pancreatic tail group, spleen and gastric invasion were dominant, leading to retroperitoneal lymph nodes metastasis. Positive immunohistochemical staining of cytokeratin and negative staining of vimentin and chromogranin A confirmed that the orthotopic pancreatic tumor injected with Pan02 cells suspended in Matrigel was of epithelial origin and expressed exocrine markers of cancer. CONCLUSION: This method of low-temperature Matrigel suspension and injection is effective for establishing an orthotopic mouse model of pancreatic cancer.
Entities:
Keywords:
C57BL/6 mouse; Matrigel; Orthotopic mouse model; Pan02; Pancreatic cancer
Authors: Collin Jacobs; Peter Duewell; Klaus Heckelsmiller; Jiwu Wei; Franz Bauernfeind; Jonathan Ellermeier; Ulrich Kisser; Christian A Bauer; Marc Dauer; Andreas Eigler; Eugene Maraskovsky; Stefan Endres; Max Schnurr Journal: Int J Cancer Date: 2011-02-15 Impact factor: 7.396
Authors: Mallika Singh; Anthony Lima; Rafael Molina; Patricia Hamilton; Anne C Clermont; Vidusha Devasthali; Jennifer D Thompson; Jason H Cheng; Hani Bou Reslan; Calvin C K Ho; Timothy C Cao; Chingwei V Lee; Michelle A Nannini; Germaine Fuh; Richard A D Carano; Hartmut Koeppen; Ron X Yu; William F Forrest; Gregory D Plowman; Leisa Johnson Journal: Nat Biotechnol Date: 2010-05-23 Impact factor: 54.908
Authors: Antonio Jimeno; Georg Feldmann; Ana Suárez-Gauthier; Zeshaan Rasheed; Anna Solomon; Gang-Ming Zou; Belen Rubio-Viqueira; Elena García-García; Fernando López-Ríos; William Matsui; Anirban Maitra; Manuel Hidalgo Journal: Mol Cancer Ther Date: 2009-01-27 Impact factor: 6.261
Authors: Sonja Gillen; Tibor Schuster; Christian Meyer Zum Büschenfelde; Helmut Friess; Jörg Kleeff Journal: PLoS Med Date: 2010-04-20 Impact factor: 11.069
Authors: Kyoko Kojima; Selwyn M Vickers; N Volkan Adsay; Nirag C Jhala; Hyung-Gyoon Kim; Trenton R Schoeb; William E Grizzle; Christopher A Klug Journal: Cancer Res Date: 2007-09-01 Impact factor: 12.701
Authors: Masakazu Hashimoto; John David Konda; Stephanie Perrino; Maria Celia Fernandez; Andrew M Lowy; Pnina Brodt Journal: Mol Cancer Ther Date: 2021-09-22 Impact factor: 6.009
Authors: Nivedita M Ratnam; Jennifer M Peterson; Erin E Talbert; Katherine J Ladner; Priyani V Rajasekera; Carl R Schmidt; Mary E Dillhoff; Benjamin J Swanson; Ericka Haverick; Raleigh D Kladney; Terence M Williams; Gustavo W Leone; David J Wang; Denis C Guttridge Journal: J Clin Invest Date: 2017-09-11 Impact factor: 14.808
Authors: Mercedes Rubio-Manzanares Dorado; Luis Miguel Marín Gómez; Daniel Aparicio Sánchez; Sheila Pereira Arenas; Juan Manuel Praena-Fernández; Juan Jose Borrero Martín; Francisco Farfán López; Miguel Ángel Gómez Bravo; Jordi Muntané Relat; Javier Padillo Ruiz Journal: World J Gastroenterol Date: 2018-02-21 Impact factor: 5.742
Authors: Lai Nar Tung; Senchuan Song; Kin Tak Chan; Mei Yuk Choi; Ho Yu Lam; Chung Man Chan; Zhiyong Chen; Hector K Wang; Hoi Ting Leung; Simon Law; Yanmin Huang; Huacan Song; Nikki P Lee Journal: Cancer Res Treat Date: 2018-01-24 Impact factor: 4.679
Authors: Simon Milette; Masakazu Hashimoto; Stephanie Perrino; Shu Qi; Michely Chen; Boram Ham; Ni Wang; Roman Istomine; Andrew M Lowy; Ciriaco A Piccirillo; Pnina Brodt Journal: Nat Commun Date: 2019-12-17 Impact factor: 14.919
Authors: Xinxia Zhu; Kevin G Burfeind; Katherine A Michaelis; Theodore P Braun; Brennan Olson; Katherine R Pelz; Terry K Morgan; Daniel L Marks Journal: J Cachexia Sarcopenia Muscle Date: 2019-01-21 Impact factor: 12.910