Asayo Imaoka1, Lin Zhang1, Noboru Kuboyama2, Yoshimitsu Abiko1. 1. Department of Biochemistry, Nihon University School of Dentistry at Matsudo. 2. Department of Pharmacology, Nihon University School of Dentistry at Matsudo.
Abstract
BACKGROUND: Low-level laser is being evaluated for treating rheumatoid arthritis (RA). Recently, the linear polarized infrared light (Super Lizer, SL) irradiation may also be useful for RA treatment. However, the molecular mechanism underlying the effectiveness of SL on RA is unclear. It has been IL-20 may involved in RA disease progression. AIM: To understand how SL action, we constructed the experimental model in vitro using human rheumatoid fibroblast-like synoviocyte (MH7A) and collagen induced (CIA) RA rat in vivo. We examined the effect of SL irradiation on IL-20 gene expression in MH7A and IL-20 protein production in CIA) rat joints. MATERIALS AND METHODS: MH7A was cultured and challenged with IL-1ß, then examined IL-20 and IL-20R mRNA level by DNA microarray. IL-20 protein expression was examined by immunohistochemistry using a specific antibody against rat IL-20. RESULT: Scatter plot analysis demonstrated that an increase in IL-20 gene expression by IL-1ß was reduced by SL irradiation, but IL-20R did not show a significant change. The Immunohistochemical analysis demonstrated a strong IL-20 staining in synovial membrane tissue of CIA rat joint, and SL irradiation significantly reduced the staining. DISCUSSION: Since IL-20 has been identified as an important cytokine in the pathogenesis of RA, the reduction of IL-20 expression by SL irradiation may be one of mechanisms in reduction of inflammation in RA joints by SL irradiation suggesting that SL irradiation may be useful for RA therapy.
BACKGROUND: Low-level laser is being evaluated for treating rheumatoid arthritis (RA). Recently, the linear polarized infrared light (Super Lizer, SL) irradiation may also be useful for RA treatment. However, the molecular mechanism underlying the effectiveness of SL on RA is unclear. It has been IL-20 may involved in RA disease progression. AIM: To understand how SL action, we constructed the experimental model in vitro using humanrheumatoid fibroblast-like synoviocyte (MH7A) and collagen induced (CIA) RA rat in vivo. We examined the effect of SL irradiation on IL-20 gene expression in MH7A and IL-20 protein production in CIA) rat joints. MATERIALS AND METHODS: MH7A was cultured and challenged with IL-1ß, then examined IL-20 and IL-20R mRNA level by DNA microarray. IL-20 protein expression was examined by immunohistochemistry using a specific antibody against ratIL-20. RESULT: Scatter plot analysis demonstrated that an increase in IL-20 gene expression by IL-1ß was reduced by SL irradiation, but IL-20R did not show a significant change. The Immunohistochemical analysis demonstrated a strong IL-20 staining in synovial membrane tissue of CIA rat joint, and SL irradiation significantly reduced the staining. DISCUSSION: Since IL-20 has been identified as an important cytokine in the pathogenesis of RA, the reduction of IL-20 expression by SL irradiation may be one of mechanisms in reduction of inflammation in RA joints by SL irradiation suggesting that SL irradiation may be useful for RA therapy.
Entities:
Keywords:
IL-20; Rheumatoid arthritis; linear polarized infrared light
Authors: Solange Almeida Dos Santos; Marcia Ataize Dos Santos Vieira; Maira Cécilia Brandão Simões; Andrey Jorge Serra; Ernesto Cesar Leal-Junior; Paulo de Tarso Camillo de Carvalho Journal: Lasers Med Sci Date: 2017-04-21 Impact factor: 3.161
Authors: Mohammed A Hadis; Siti A Zainal; Michelle J Holder; James D Carroll; Paul R Cooper; Michael R Milward; William M Palin Journal: Lasers Med Sci Date: 2016-03-10 Impact factor: 3.161