Literature DB >> 25070342

Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs.

Hung Nguyen1, Nima Badie, Luke McSpadden, Dawn Pedrotty, Nenad Bursac.   

Abstract

Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of cell-cell contact. These cell pairs can be utilized in patch clamp recordings to quantify electrical interactions between cardiomyocytes and non-cardiomyocytes.

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Year:  2014        PMID: 25070342      PMCID: PMC4219610          DOI: 10.1007/978-1-4939-1047-2_21

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  19 in total

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  3 in total

1.  Generation and customization of biosynthetic excitable tissues for electrophysiological studies and cell-based therapies.

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