| Literature DB >> 2506940 |
D V Apte1, Y Koutalos, D K McFarlane, M J Dawson, T G Ebrey.
Abstract
Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spectra of whole retinae. The intracellular pH was determined to be 7.27 +/- 0.06 at 4 degrees C and the intracellular MgNTP/NTP ratio was at least 0.77. These results are consistent with those reported by other techniques, and they show that 31P-NMR spectroscopy can be used for noninvasively and quantitatively studying the metabolism of living toad retinae, and for monitoring its changes over time.Entities:
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Year: 1989 PMID： 2506940 PMCID： PMC1280497 DOI： 10.1016/S0006-3495(89)82691-8
Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033