M J Koehler1, K Kellner, U-C Hipler, M Kaatz. 1. Department of Dermatology, SRH Waldklinikum Gera, Gera, Germany; Department of Dermatology, University Hospital Jena, Jena, Germany.
Abstract
BACKGROUND: In vivo multiphoton tomography (MPT) of human skin has become a valuable tool for non-invasive examination of morphological and biophysical skin properties and their alterations. So far, skin changes after UVB irradiation were mainly evaluated clinically and histologically. The present study aimed at non-invasive imaging of histological changes during acute UVB irradiation by multiphoton laser tomography. METHODS: In 10 volunteers, five areas were irradiated once with an erythematous UVB dose. Multiphoton measurements were performed four times, i.e. before irradiation (baseline), and 24, 48 and 72 h after irradiation, respectively. The data were evaluated for changes of epidermal pleomorphy, spongiosis, pigmentation and thickness. RESULTS: The four parameters were altered significantly by acute UVB irradiation, i.e. epidermal pleomorphy, spongiosis, pigmentation and thickness increased within 72 h after irradiation. CONCLUSION: Thus, the study has shown that typical epidermal changes induced by acute UVB irradiation can be evaluated by MPT.
BACKGROUND: In vivo multiphoton tomography (MPT) of human skin has become a valuable tool for non-invasive examination of morphological and biophysical skin properties and their alterations. So far, skin changes after UVB irradiation were mainly evaluated clinically and histologically. The present study aimed at non-invasive imaging of histological changes during acute UVB irradiation by multiphoton laser tomography. METHODS: In 10 volunteers, five areas were irradiated once with an erythematous UVB dose. Multiphoton measurements were performed four times, i.e. before irradiation (baseline), and 24, 48 and 72 h after irradiation, respectively. The data were evaluated for changes of epidermal pleomorphy, spongiosis, pigmentation and thickness. RESULTS: The four parameters were altered significantly by acute UVB irradiation, i.e. epidermal pleomorphy, spongiosis, pigmentation and thickness increased within 72 h after irradiation. CONCLUSION: Thus, the study has shown that typical epidermal changes induced by acute UVB irradiation can be evaluated by MPT.
Authors: Jonas Wizenty; Teresa Schumann; Donna Theil; Martin Stockmann; Johann Pratschke; Frank Tacke; Felix Aigner; Tilo Wuensch Journal: Molecules Date: 2020-04-30 Impact factor: 4.411