Mohammad Naderi1, Mohammad Hashemi2, Zahra Pourmontaseri1, Ebrahim Eskandari-Nasab3, Gholamreza Bahari4, Mohsen Taheri5. 1. Research Center for Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. 2. Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran; Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. Electronic address: mhd.hashemi@gmail.com. 3. Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. 4. Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. 5. Genetics of Non-Communicable Disease Research Centre, Zahedan University of Medical Sciences, Zahedan, Iran.
Abstract
OBJECTIVE: To evaluate the possible association between Toll-interleukin 1 receptor (TIR) domain containing adaptor protein (TIRAP; also known as MAL) rs1893352 and rs8177374 (S180L) gene polymorphisms and pulmonary tuberculosis (PTB) in a sample of Iranian population. METHODS: This case-control study was performed on 174 PTB and 177 healthy subjects. Tetra amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) was used to detect the polymorphisms. RESULTS: Our finding showed that neither the overall Chi-square comparison of PTB and control subjects nor the logistic regression analysis indicated any association between rs1893352 polymorphism and PTB. Regarding rs8177374 polymorphism, the CT genotype as well as CT+TT increased the risk of PTB in comparison with CC genotype (OR=4.73, 95% CI=2.65-8.45, P<0.0001 and OR=6.47, 95% CI=3.68-11.38, P<0.0001, respectively). The rs8177374 T allele increased the risk of PTB in comparison with C allele (OR=4.21, 95% CI=2.43-7.26, P<0.0001). CONCLUSIONS: Our finding indicates that TIRAP rs8177374 polymorphism is associated with PTB in a sample of Iranian population.
OBJECTIVE: To evaluate the possible association between Toll-interleukin 1 receptor (TIR) domain containing adaptor protein (TIRAP; also known as MAL) rs1893352 and rs8177374 (S180L) gene polymorphisms and pulmonary tuberculosis (PTB) in a sample of Iranian population. METHODS: This case-control study was performed on 174 PTB and 177 healthy subjects. Tetra amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) was used to detect the polymorphisms. RESULTS: Our finding showed that neither the overall Chi-square comparison of PTB and control subjects nor the logistic regression analysis indicated any association between rs1893352 polymorphism and PTB. Regarding rs8177374 polymorphism, the CT genotype as well as CT+TT increased the risk of PTB in comparison with CC genotype (OR=4.73, 95% CI=2.65-8.45, P<0.0001 and OR=6.47, 95% CI=3.68-11.38, P<0.0001, respectively). The rs8177374 T allele increased the risk of PTB in comparison with C allele (OR=4.21, 95% CI=2.43-7.26, P<0.0001). CONCLUSIONS: Our finding indicates that TIRAP rs8177374 polymorphism is associated with PTB in a sample of Iranian population.