Héctor M Almau Trenard1, Luis E Moulin1, Juan M Padín1, Pablo Stringa2, Gabriel E Gondolesi3, Pablo Barros Schelotto4. 1. Instituto de Trasplante Multiorgánico, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina. 2. Laboratorio de Microcirugía Experimental, CONICET, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina. 3. Instituto de Trasplante Multiorgánico, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina; Laboratorio de Microcirugía Experimental, CONICET, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina. 4. Instituto de Trasplante Multiorgánico, Hospital Universitario, Fundación Favaloro, Buenos Aires, Argentina. Electronic address: pbarros@ffavaloro.org.
Abstract
BACKGROUND: Liver failure might be a cause of death after major hepatectomies. The ALPPS technique appears to be a promising strategy to avoid it, however no experimental studies supporting this procedure have been previously described. The aim was to develop an experimental model of ALPPS in rats. METHOD: Experimental. A total of 30 Sprague Dawley rats were used. To develop the ALPPS procedure, ligation of the left portal branch of the middle lobe (LM) was performed. This demarcates the left side (SILM) from the right side (SDLM); parenchyma transection was performed following the demarcated line. The animal's weight, volume and weight of both LM were analyzed. Sacrifice at 3, 7 and 14 days after the procedure (10 per group) was performed. RESULTS: No bleeding or ascites were observed during the postoperative period. The LM increased by 24.1, 86.9 and 120.4% at 3, 7 and 14 days. The SDLM increased by 34.4, 78.8 and 102.0% at 3, 7 and 14 days. The SILM decreased 42.6, 64.8, and 79.3% at day 3, 7 and 14 days respectively. CONCLUSION: The ALPPS procedure can be performed in rats, achieving the expected results. Comparison studies to 2 staged hepatectomy will be necessary.
BACKGROUND:Liver failure might be a cause of death after major hepatectomies. The ALPPS technique appears to be a promising strategy to avoid it, however no experimental studies supporting this procedure have been previously described. The aim was to develop an experimental model of ALPPS in rats. METHOD: Experimental. A total of 30 Sprague Dawley rats were used. To develop the ALPPS procedure, ligation of the left portal branch of the middle lobe (LM) was performed. This demarcates the left side (SILM) from the right side (SDLM); parenchyma transection was performed following the demarcated line. The animal's weight, volume and weight of both LM were analyzed. Sacrifice at 3, 7 and 14 days after the procedure (10 per group) was performed. RESULTS: No bleeding or ascites were observed during the postoperative period. The LM increased by 24.1, 86.9 and 120.4% at 3, 7 and 14 days. The SDLM increased by 34.4, 78.8 and 102.0% at 3, 7 and 14 days. The SILM decreased 42.6, 64.8, and 79.3% at day 3, 7 and 14 days respectively. CONCLUSION: The ALPPS procedure can be performed in rats, achieving the expected results. Comparison studies to 2 staged hepatectomy will be necessary.
Authors: Erik Schadde; Martin Hertl; Stefan Breitenstein; Beatrice Beck-Schimmer; Martin Schläpfer Journal: J Vis Exp Date: 2017-08-14 Impact factor: 1.355