Yousef Dadimoghaddam1, Ahmad Daryani2, Mehdi Sharif1, Ehsan Ahmadpour3, Zahra Hossienikhah4. 1. Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Parasitology and Mycology Department, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran. 2. Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Parasitology and Mycology Department, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran. Electronic address: daryanii@yahoo.com. 3. Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Parasitology and Mycology Department, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran. Electronic address: ehsanahmadpour@gmail.com. 4. Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract
OBJECTIVE: To evaluate parasite distribution and tissue tropism of Toxoplasma gondii tachyzoites in experimentally infected mice using real time QPCR. METHODS: In this survey 16 Balb/c mice were inoculated with 1 × 10(4) alive tachyzoites of Toxoplasma gondii RH strain. After 1, 2, 3 days post infection and the last day (before death), different tissues of mice including blood, brain, eye, liver, spleen, kidney, heart and muscle were harvested. Following tissues DNA extraction, the parasite burden was quantified using real time QPCR targeting the B1 gene (451 bp). RESULTS: It showed that Toxoplasma after intraperitoneal injection was able to movement to various tissues in 24 hours. Parasite burden was high in all tissues but the most number of parasites were observed in kidney, heart and liver, respectively. CONCLUSIONS: These data provide significant baseline information about Toxoplasma pathogenesis, vaccine monitoring and drug efficiency.
OBJECTIVE: To evaluate parasite distribution and tissue tropism of Toxoplasma gondii tachyzoites in experimentally infected mice using real time QPCR. METHODS: In this survey 16 Balb/c mice were inoculated with 1 × 10(4) alive tachyzoites of Toxoplasma gondii RH strain. After 1, 2, 3 days post infection and the last day (before death), different tissues of mice including blood, brain, eye, liver, spleen, kidney, heart and muscle were harvested. Following tissues DNA extraction, the parasite burden was quantified using real time QPCR targeting the B1 gene (451 bp). RESULTS: It showed that Toxoplasma after intraperitoneal injection was able to movement to various tissues in 24 hours. Parasite burden was high in all tissues but the most number of parasites were observed in kidney, heart and liver, respectively. CONCLUSIONS: These data provide significant baseline information about Toxoplasma pathogenesis, vaccine monitoring and drug efficiency.
Authors: Fatemeh Rezaei; Mohammad Ali Ebrahimzadeh; Ahmad Daryani; Mehdi Sharif; Ehsan Ahmadpour; Shahabeddin Sarvi Journal: J Parasit Dis Date: 2014-12-20
Authors: Luiz Carlos De Mattos; Ana Iara Costa Ferreira; Karina Younan de Oliveira; Fabiana Nakashima; Cinara Cássia Brandão Journal: Front Cell Infect Microbiol Date: 2021-06-18 Impact factor: 5.293