Hossein Hamidinejat1, Mohammad Hossein Razi Jalali2, Ramezan Ali Jafari3, Kiomars Nourmohammadi4. 1. Department of Pathobiology, Veterinary Faculty, Shahid Chamran University, Ahvaz 145-61355, Iran. Electronic address: hamidinejat@yahoo.com. 2. Department of Pathobiology, Veterinary Faculty, Shahid Chamran University, Ahvaz 145-61355, Iran. 3. Department of Clinical Sciences, Veterinary Faculty, Shahid Chamran University, Ahvaz 145-61355, Iran. 4. MSc Student of Parasitology, Shahid Chamran University, Ahvaz 145-61355, Iran.
Abstract
OBJECTIVE: To identify the genotypes of prevalent Cryptosporidia in broiler chickens in Lorestan province, Iran. METHODS: A total of 1 000 fecal and 1 000 trachea samples were collected from chickens. Smears from both fecal and tracheal samples were stained with modified Ziehl-Neelsen method and nested PCR-RFLP according to amplification of 18S rRNA gene using Ssp I and Vsp I restriction enzymes and DNA sequencing. RESULTS: From the examined chickens 0.7% was positive for Cryptosporidium. Infection was present in 0.5% fecal samples and also in 0.5% trachea. Only 0.3% of simultaneous infections in fecal and tracheal samples were observed. Nested PCR of our isolates demonstrated Cryptosporidium baileyi. CONCLUSIONS: In our work, low rate of Cryptosporidium baileyi infection was detected, but in critical situations and our poor management circumstances, cryptosporidiosis occurs in serious feature especially in immune suppressed individuals.
OBJECTIVE: To identify the genotypes of prevalent Cryptosporidia in broiler chickens in Lorestan province, Iran. METHODS: A total of 1 000 fecal and 1 000 trachea samples were collected from chickens. Smears from both fecal and tracheal samples were stained with modified Ziehl-Neelsen method and nested PCR-RFLP according to amplification of 18S rRNA gene using Ssp I and Vsp I restriction enzymes and DNA sequencing. RESULTS: From the examined chickens 0.7% was positive for Cryptosporidium. Infection was present in 0.5% fecal samples and also in 0.5% trachea. Only 0.3% of simultaneous infections in fecal and tracheal samples were observed. Nested PCR of our isolates demonstrated Cryptosporidium baileyi. CONCLUSIONS: In our work, low rate of Cryptosporidium baileyi infection was detected, but in critical situations and our poor management circumstances, cryptosporidiosis occurs in serious feature especially in immune suppressed individuals.
Authors: Alireza Zahedi; Andrea Paparini; Fuchun Jian; Ian Robertson; Una Ryan Journal: Int J Parasitol Parasites Wildl Date: 2015-12-12 Impact factor: 2.674