Chu-Hang Liao1, Wei Fei1, Zhi-Hao Shen1, Ming-Ping Yin1, Chen Lu2. 1. Oral and Maxillofacial Surgery of Sichuan Provincial Academy of Medical Sciences, Sichuan Provincial People's Hospital 610072, Chengdu, China. 2. Chengdu Medical College 610083, Chengdu, China.
Abstract
OBJECTIVE: To simulate the expression of TNF-α and PGE2 of periodontal tissues in rat periodontitis model. METHODS: 40 Wistar rats were randomly divided into the periodontitis group and the control group (n=20). After the successful establishment of periodontitis rat model, raising for six weeks before the animals were sacrificed. The periodontal tissues were obtained and made into slices. Observed the histopathological changes of the periodontal tissues and measured TNF-α, PGE2 levels change by immunohistochemistry, Western blot analysis and ELISA. RESULTS: TNF-α, PGE2 expression of the periodontitis group was significantly higher than that in the control group, the difference was significant (P<0.05). CONCLUSIONS: The TNF-α, PGE2 expression of the rat periodontal tissue in the periodontitis group was significantly higher than the control group.
OBJECTIVE: To simulate the expression of TNF-α and PGE2 of periodontal tissues in ratperiodontitis model. METHODS: 40 Wistar rats were randomly divided into the periodontitis group and the control group (n=20). After the successful establishment of periodontitisrat model, raising for six weeks before the animals were sacrificed. The periodontal tissues were obtained and made into slices. Observed the histopathological changes of the periodontal tissues and measured TNF-α, PGE2 levels change by immunohistochemistry, Western blot analysis and ELISA. RESULTS: TNF-α, PGE2 expression of the periodontitis group was significantly higher than that in the control group, the difference was significant (P<0.05). CONCLUSIONS: The TNF-α, PGE2 expression of the rat periodontal tissue in the periodontitis group was significantly higher than the control group.
Authors: Francisco Isaac Fernandes Gomes; Maria Gerusa Brito Aragão; Francisco Cesar Barroso Barbosa; Mirna Marques Bezerra; Vicente de Paulo Teixeira Pinto; Hellíada Vasconcelos Chaves Journal: J Oral Maxillofac Res Date: 2016-06-30
Authors: Alrieta H Teixeira; Jordânia M de Oliveira Freire; Luzia H T de Sousa; Antônia T Parente; Nayara A de Sousa; Angela M C Arriaga; Francisca R Lopes da Silva; Iracema M Melo; Igor I Castro da Silva; Karuza M A Pereira; Paula Goes; José J do Nascimento Costa; Gerardo Cristino-Filho; Vicente de Paulo T Pinto; Hellíada V Chaves; Mirna M Bezerra Journal: Front Physiol Date: 2017-12-01 Impact factor: 4.566